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PMID: 6221717 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Differential effects of polyamines on the phosphorylation of chromatin-associated proteins.

The Biochemical journal ·Vol. 209 ·No. 1 ·1983-01-01 ·Pages 197-205

Ahmed K, Davis AT, Goueli SA

Abstract

Studies are presented on the nature of chromatin-associated phosphoproteins whose phosphorylation is influenced by polyamines. After labelling with 32P, chromatin-associated proteins were separated into four fractions. Fraction I comprised neutral and basic non-histone phosphoproteins, including high-mobility-group non-histones; fraction II consisted mostly of histones; fraction III consisted of a class of (salt-soluble) acidic non-histone phosphoproteins; and fraction IV consisted of residual (salt-insoluble) acidic non-histone phosphoproteins. The average relative distribution of protein in the four fractions (I-IV) was about 1:4:2:1 for both liver and prostate. However, tissue-dependent differences were observed in the incorporation of 32P in various protein fractions. In the presence of polyamines (e.g. 1 mM-spermine or 2 mM-spermidine) maximal stimulation of phosphorylation was observed in non-histone proteins of fraction I (160-180%), followed by that in non-histone proteins of fraction III (80-110%). The phosphorylation of residual non-histone proteins in fraction IV, and the small extent of phosphorylation of histones in fraction II, remained unaltered in the presence of polyamines. Thus polyamines do not stimulate the phosphorylation of all non-histone proteins; their stimulative effect is most prominent in the phosphorylation of neutral and basic non-histone proteins and a class of salt-soluble acidic non-histone proteins. In accord with our hypothesis, these differential effects of polyamines on phosphorylation of endogenous non-histone proteins may relate to the conformation of these substrates rather than to endogenous kinases.

MeSH Terms
Animals Chromatin/drug effects,metabolism Chromosomal Proteins, Non-Histone/metabolism Electrophoresis, Polyacrylamide Gel High Mobility Group Proteins Histones/metabolism Liver/metabolism Male Phosphorylation Polyamines/pharmacology Prostate/metabolism Rats Rats, Inbred Strains
Chemicals
Chromatin Chromosomal Proteins, Non-Histone High Mobility Group Proteins Histones Polyamines
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ahmed K
Davis A T
Goueli S A
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31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2090197
Published
1983-01-01
Pages
197-205
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1154072
Subset
IM
Grants
NCI NIH HHS · R01 CA015062 · United States
NCI NIH HHS · CA 15062 · United States
External Links
PubMed source DOI: 10.1042/bj2090197 PMC Full Text View journal details
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