Home LiteratureArticle Details
PMID: 6221801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Opsin-immunoreactive outer segments in the pineal and parapineal organs of the lamprey (Lampetra fluviatilis), the eel (Anguilla anguilla), and the rainbow trout (Salmo gairdneri).

Cell and tissue research ·Vol. 230 ·No. 2 ·1983-00-00 ·Pages 289-307

Vigh-Teichmann I, Korf HW, Nürnberger F, Oksche A, Vigh B, Olsson R

Abstract

The pineal complex of Lampetra fluviatilis, Anguilla anguilla and Salmo gairdneri was studied by means of the indirect immunohistochemical antiopsin reaction. Opsin-immunoreactive material was demonstrated in the outer segments of the photoreceptor cells in the pineal organ of all three species investigated. In the lamprey, the opsin-positive outer segments were located in the lumen of the pineal vesicle and atrium. In the two teleost species, the immunoreactive outer segments were observed in abundance in the pineal end-vesicle and stalk. These structures were found to accumulate in the prominent initial portion of the pineal stalk of the eel. In the rainbow trout, immunoreactive outer segments occurred in the wide orifice of the pineal recess at the roof of the third ventricle. In addition, outer segments of photoreceptor cells of the parapineal organ ("parapinealocytes") displayed opsin immunoreactivity. In the lamprey, opsin immunoreactivity was restricted to the central portion of the ventral parapineal retina, while the parapinealocytes in the lateral portions did not bind the antibody. In the two teleosts, immunoreactive outer segments displayed a scattered pattern. These immunocytochemical results provide direct evidence that the photosensitivity of the pineal demonstrated electrophysiologically in lampreys and teleosts (cf. Dodt 1973) is based on an opsin-containing photopigment. The presence of opsin in cells of the parapineal organ strengthens the view that also this organ may be capable of direct light perception. In the lamprey, the exclusive opsin immunoreactivity of a circumscribed group of parapineal cells suggests the existence of two types of parapinealocytes. The significance of opsin-containing photoreceptor outer segments occurring in the most proximal portion of the teleost pineal stalk is discussed, especially with regard to the interpretation of results obtained from pinealectomy experiments.

MeSH Terms
Animals Eels Eye Proteins/analysis Female Immunoenzyme Techniques Lampreys Male Photoreceptor Cells/cytology Pineal Gland/anatomy & histology Rod Cell Outer Segment/analysis Rod Opsins Trout
Chemicals
Eye Proteins Rod Opsins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vigh-Teichmann I
Korf H W
Nürnberger F
Oksche A
Vigh B
Olsson R
References (59)
59 references, click to expand
  1. Responses of pineal photoreceptors in the brook and rainbow trout.
    Cell Tissue Res. 1980;208(1):111-22 PMID: 7190070
  2. Localization of rhodopsin in isolated, osmotically intact rod outer segment discs.
    J Histochem Cytochem. 1979 Mar;27(3):788-93 PMID: 314460
  3. [Cytophysiological study of the parapineal organ of Lamperta laneri].
    J Neurovisc Relat. 1971;32(3):157-99 PMID: 4329007
  4. PHOTOSENSITIVITY OF THE PINEAL ORGAN IN THE TELEOST, SALMO IRIDEUS (GIBBONS).
    Experientia. 1963 Dec 15;19:642-3 PMID: 14099867
  5. The visual pigments of freshwater fishes.
    Vision Res. 1967 Mar;7(3):121-48 PMID: 5613289
  6. Structure of the parapineal organ of the adult rainbow trout, Salmo gairdneri Richardson.
    Z Zellforsch Mikrosk Anat. 1969;93(2):282-304 PMID: 4905352
  7. Pineal photosensitivity in three teleosts, Salmo irideus, Plecoglossus altivelis and Mugil cephalus.
    Rev Can Biol. 1970 Jun;29(2):133-40 PMID: 5504297
  8. Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells. An electron microscope analysis of cross-linked albumin-embedded retinas.
    J Cell Biol. 1978 Apr;77(1):196-210 PMID: 350891
  9. A simple method for removing the resin from epoxy-embedded tissue.
    J Biophys Biochem Cytol. 1961 Apr;9:909-10 PMID: 13768615
  10. Vitamin A1/A2-based visual pigment mixtures in cones of the rudd.
    Vision Res. 1976;16(9):891-6 PMID: 948877
  11. Properties of the epiphysis cerebri of the small-spotted dogfish shark, Scyliorhinus caniculus L.
    Vision Res. 1971 Mar;11(3):189-98 PMID: 5579835
  12. Origin of the slow potential in the pineal organ of the rainbow trout.
    Vision Res. 1975 Jun;15(6):737-40 PMID: 1138492
  13. Membrane protein analysis by two-dimensional immunoelectrophoresis.
    Science. 1975 Aug 8;189(4201):469-72 PMID: 1154021
  14. Anatomical and physiological characteristics of pineal photoreceptor cell in the larval lamprey, Petromyzon marinus.
    J Neurophysiol. 1981 Nov;46(5):1018-38 PMID: 7299444
  15. Pineal-gonad relationships in the lamprey Lampetra fluviatilis.
    Gen Comp Endocrinol. 1973 Aug;21(1):118-22 PMID: 4724756
  16. Ultrastructure of serotonin-containing cells in the pineal organ of Lampetra planeri (Petromyzontidae).
    Cell Tissue Res. 1980;207(3):407-27 PMID: 7397755
  17. Polarized microspectrophotometry for pigment orientation and concentration.
    Methods Enzymol. 1982;81:642-7 PMID: 7098904
  18. Pineal complex of the clawed toad, Xenopus laevis Daud.: structure and function.
    Cell Tissue Res. 1981;216(1):113-30 PMID: 7226202
  19. The pineal organ of Raja clavata: opsin immunoreactivity and ultrastructure.
    Cell Tissue Res. 1983;228(1):139-48 PMID: 6219741
  20. Morphology of the pineal complex of the anadromous sea lamprey, Petromyzon marinus L.
    Am J Anat. 1982 Oct;165(2):131-63 PMID: 7148728
  21. Immunoreactive opsin in the pineal organ of reptiles and birds.
    Z Mikrosk Anat Forsch. 1982;96(1):113-29 PMID: 6213109
  22. [Scanning electron microscopy study of pineal photoreceptor cells in the trout, Salmo gairdneri (Teleostei)].
    Z Zellforsch Mikrosk Anat. 1973;138(4):585-9 PMID: 4718161
  23. Morphology of vertebrate photoreceptors.
    Methods Enzymol. 1982;81:3-17 PMID: 7098873
  24. Opsin-immunoreactive outer segments and acetylcholinesterase-positive neurons in the pineal complex of Phoxinus phoxinus (Teleostei, Cyprinidae).
    Cell Tissue Res. 1982;227(2):351-69 PMID: 6217894
  25. [COMPARATIVE ELECTRON MICROSCOPIC STUDIES OF THE PINEAL BODY].
    Prog Brain Res. 1965;10:237-58 PMID: 14281605
  26. The eel retina. Receptor classes and spectral mechanisms.
    J Gen Physiol. 1978 Feb;71(2):123-38 PMID: 641517
  27. Light and electron microscopic studies on the pineal tract of rainbow trout, Salmo gairdneri.
    Rev Can Biol. 1979 Jun;38(2):105-18 PMID: 493566
  28. Studies on central projections of the pineal nerve tract in rainbow trout, Salmo gairdneri Richardson, using cobalt chloride iontophoresis.
    Cell Tissue Res. 1974;154(4):485-510 PMID: 4140765
  29. [Lead pattern of the pineal neuron of the rainbow trout (Salmo irideus) by illumination of the diencephalon].
    Pflugers Arch Gesamte Physiol Menschen Tiere. 1966;289(3):155-67 PMID: 5237284
  30. Structure of the pineal organs of Anguilla anguilla L. and Lebistes reticulatus Peters (Teleostei).
    Z Zellforsch Mikrosk Anat. 1971;122(2):227-43 PMID: 4330408
  31. Innervation of the vertebrate pineal.
    Prog Brain Res. 1979;52:45-88 PMID: 398536
  32. Rhodopsin biosynthesis in isolated retinas.
    Methods Enzymol. 1982;81:772-82 PMID: 7047996
  33. Rod and cone visual pigments in the goldfish.
    Vision Res. 1981;21(6):943-6 PMID: 7314473
  34. [The pineal complex of the ammocoete (Lampetra planeri). Connections of the pineal and parapineal organs with the epithalamic roof].
    Z Zellforsch Mikrosk Anat. 1971;117(3):354-80 PMID: 4326435
  35. Comparative study of a membrane protein. Characterization of bovine, rat, and frog visual pigments500.
    Biochemistry. 1969 Feb;8(2):675-9 PMID: 5793717
  36. The role of the pineal complex in the pigmentary effector system of the lampreys, Mordacia mordax (Richardson) and Geotria australis Gray.
    Gen Comp Endocrinol. 1968 Dec;11(3):528-34 PMID: 5726272
  37. Neurobiological aspects of extraretinal photoreceptive systems: structure and function.
    Experientia. 1982 Sep 15;38(9):991-6 PMID: 6751862
  38. [Ganglion cells and the pineal body of Lampetra planeri].
    J Neurovisc Relat. 1969;31(3):308-33 PMID: 5364100
  39. Cone structure and visual pigment content in the retina of the goldfish.
    Vision Res. 1976;16(6):647-57 PMID: 960588
  40. The parapineal and pineal organs of the elver (glass eel), Anguilla anguilla L.
    Cell Tissue Res. 1982;222(2):433-44 PMID: 7083311
  41. Photoreceptor membrane carbohydrate on the intradiscal surface of retinal rod disks.
    Nature. 1976 Oct 28;263(5580):789-91 PMID: 1086986
  42. Biosynthetic and immunochemical characterization of large protein in frog and cattle rod outer segment membranes.
    Exp Eye Res. 1976 Aug;23(2):105-15 PMID: 976361
  43. The ecology of cone pigments in teleost fishes.
    Vision Res. 1978;18(6):715-22 PMID: 664357
  44. Comparative ultrastructure of cerebrospinal fluid-contacting neurons and pinealocytes.
    Cell Tissue Res. 1975;158(3):409-24 PMID: 807327
  45. Comparison of the pineal complex, retina and cerebrospinal fluid contacting neurons by immunocytochemical antirhodopsin reaction.
    Z Mikrosk Anat Forsch. 1980;94(4):623-40 PMID: 7456628
  46. Light- and electron-microscopic demonstration of immunoreactive opsin in the pinealocytes of various vertebrates.
    Cell Tissue Res. 1981;221(2):451-63 PMID: 6458362
  47. Nervous connections of the parietal eye in adult Lacerta s. sicula Rafinesque as demonstrated by anterograde and retrograde transport of horseradish peroxidase.
    Cell Tissue Res. 1981;219(3):567-83 PMID: 6168382
  48. Acetylcholinesterase-positive neurons in the pineal and parapineal organs of the rainbow trout, Salmo gairdneri (with special reference to the pineal tract).
    Cell Tissue Res. 1974;155(4):475-89 PMID: 4614911
  49. SURVEY OF THE DEVELOPMENT AND COMPARATIVE MORPHOLOGY OF THE PINEAL ORGAN.
    Prog Brain Res. 1965;10:3-29 PMID: 14281614
  50. The presence of two populations of sensory-type cells in the pineal organ of the five-bearded rockling, Ciliata mustela L. (Teleostei).
    Cell Tissue Res. 1983;230(3):553-71 PMID: 6850781
  51. [ELECTRON MICROSCOPIC STUDIES OF THE PINEAL BODY OF THE RAINBOW TROUT (SALMO IRIDEUS)].
    Prog Brain Res. 1965;10:259-69 PMID: 14281606
  52. Letter: Evidence for photosensitive pigments in the pineal complex of the frog.
    Vision Res. 1974 Jul;14(7):597-8 PMID: 4547449
  53. Histochemical and ultrastructural study of the nervous elements in the pineal organ of the eel, Anuilla anguilla.
    Cell Tissue Res. 1981;216(3):545-55 PMID: 7237520
  54. A correlation of ultrastructure and function in the developing retina oof the frog tadpole.
    J Ultrastruct Res. 1970 Jan;30(1):87-102 PMID: 5411817
  55. Synaptic ribbons in the pineal organ of the goldfish: circadian rhythmicity and the effects of constant light and constant darkness.
    Cell Tissue Res. 1981;215(3):491-7 PMID: 7214490
  56. Renewal of photoreceptor cells.
    Methods Enzymol. 1982;81:763-72 PMID: 7047995
  57. Intrinsic neurons and neural connections of the pineal organ of the house sparrow, Passer domesticus, as revealed by anterograde and retrograde transport of horseradish peroxidase.
    Cell Tissue Res. 1982;222(2):243-60 PMID: 7083302
  58. Pineal sense organs--components of photoneuroendocrine systems.
    Prog Brain Res. 1979;52:113-30 PMID: 549077
  59. Membrane biosynthesis in the frog retina: opsin transport in the photoreceptor cell.
    Biochemistry. 1975 Apr 8;14(7):1343-52 PMID: 1079139
Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00213806
Published
1983-00-00
Pages
289-307
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00213806 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2