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PMID: 6222244 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA repair properties of Escherichia coli tif-1, recAo281 and lexA1 strains deficient in single-strand DNA binding protein.

Molecular & general genetics : MGG ·Vol. 190 ·No. 1 ·1983-00-00 ·Pages 101-11

Whittier RF, Chase JW

Abstract

Mutations affecting single-strand DNA binding protein (SSB) impair induction of mutagenic (SOS) repair. To further investigate the role of SSB in SOS induction and DNA repair, isogenic strains were constructed combining the ssb+, ssb-1 or ssb-113 alleles with one or more mutations known to alter regulation of damage inducible functions. As is true in ssb+ strains tif-1 (recA441) was found to allow thermal induction of prophage lambda + and Weigle reactivation in ssb-1 and ssb-113 strains. Furthermore, tif-1 decreased the UV sensitivity of the ssb-113 strain slightly and permitted UV induction of prophage lambda + at 30 degrees C. Strains carrying the recAo281 allele were also constructed. This mutation causes high constitutive levels of RecA protein synthesis and relieves much of the UV sensitivity conferred by lexA- alleles without restoring SOS (error-prone) repair. In contrast, the recAo281 allele failed to alleviate the UV sensitivity associated with either ssb- mutation. In a lexA1 recAo281 background the ssb-1 mutation increased the extent of postirradiation DNA degradation and concommitantly increased UV sensitivity 20-fold to the level exhibited by a recA1 strain. The ssb-113 mutation also increased UV sensitivity markedly in this background but did so without greatly increasing postirradiation DNA degradation. These results suggest a direct role for SSB in recombinational repair apart from and in addition to its role in facilitating induction of the recA-lexA regulon.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda/radiation effects Carrier Proteins/genetics DNA Repair/radiation effects DNA, Bacterial/metabolism Escherichia coli/genetics,metabolism,radiation effects Mutation Rec A Recombinases Serine Endopeptidases Ultraviolet Rays Virus Activation/radiation effects
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial LexA protein, Bacteria Rec A Recombinases Serine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Whittier R F
Chase J W
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48 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330330
Published
1983-00-00
Pages
101-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · CA13330 · United States
NIGMS NIH HHS · GM11301 · United States
NIGMS NIH HHS · GM23451 · United States
External Links
PubMed source DOI: 10.1007/BF00330330
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