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PMID: 6223885 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.

Infection and immunity ·Vol. 41 ·No. 2 ·1983-08-00 ·Pages 872-5

Furuta T, Nisizawa T, Chiba J, Hamada S

Abstract

A mouse hybrid cell line secreted monoclonal antibody which reacted specifically with Streptococcus mutans 6715 (serotype g) glucosyltransferase (GTase)-synthesizing water-insoluble glucan and inhibited with enzyme reaction. The antibody was cross-reactive with GTase of serotype d but not with GTase of other serotypes of S. mutans when an enzyme-linked immunosorbent assay was used.

MeSH Terms
Animals Antibodies, Monoclonal/analysis,biosynthesis Cross Reactions Glucosyltransferases/immunology Hybridomas/immunology Mice Mice, Inbred BALB C Serotyping Streptococcus mutans/enzymology,immunology
Chemicals
Antibodies, Monoclonal Glucosyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Furuta T
Nisizawa T
Chiba J
Hamada S
References (14)
14 references, click to expand
  1. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
    J Immunol. 1972 Jul;109(1):129-35 PMID: 4113792
  2. A high resolution PAS stain for polyacrylamide gel electrophoresis.
    Anal Biochem. 1973 Dec;56(2):361-9 PMID: 4128880
  3. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  4. Production of large amounts of antibodies in individual mice.
    J Immunol. 1976 Mar;116(3):676-81 PMID: 1254948
  5. Adherence of serotype e Streptococcus mutans and the inhibitory effect of Lancefield group E and S mutans type e antiserum.
    J Dent Res. 1976 Apr;55 Spec No:C65-74 PMID: 4482
  6. Serum glucosyltransferase-inhibiting antibodies and dental caries in rhesus monkeys immunized against Streptococcus mutans.
    Immunology. 1976 May;30(5):619-27 PMID: 819360
  7. Immunological relationships between glucosyltransferases from Streptococcus mutans serotypes.
    Infect Immun. 1976 Sep;14(3):636-44 PMID: 965089
  8. Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans.
    Infect Immun. 1977 Jan;15(1):91-103 PMID: 64460
  9. Preparation of glucosyltransferase from Streptococcus mutans by elution from water-insoluble polysaccharide with a dissociating solvent.
    Infect Immun. 1979 Feb;23(2):446-52 PMID: 422248
  10. Serotype-dependent inhibition of glucan synthesis and cell adherence of Streptococcus mutans by antibody against glucosyltransferase of serotype e S. mutans.
    Microbiol Immunol. 1979;23(2):61-70 PMID: 111004
  11. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  12. Glycosyltransferases of Streptococcus mutans strain Ingbritt.
    Microbios. 1978;23(93-94):136-46 PMID: 117283
  13. Biology, immunology, and cariogenicity of Streptococcus mutans.
    Microbiol Rev. 1980 Jun;44(2):331-84 PMID: 6446023
  14. Immunochemical properties of glucosyltransferases from Streptococcus mutans.
    Infect Immun. 1983 Feb;39(2):762-6 PMID: 6187685
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
1983-08-00
Pages
872-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264727
Subset
IM
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