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PMID: 6223918 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

SOS induction by P1 Km miniplasmids.

Journal of bacteriology ·Vol. 155 ·No. 2 ·1983-08-00 ·Pages 473-80

Capage MA, Scott JR

Abstract

We have constructed (in vitro) a set of P1 miniplasmids. The smallest of these that could function as an independent replicon contained the right side of EcoRI-5 plus all of EcoRI-8. Those miniplasmids that lack EcoRI-6 induce the SOS pathway of the cell as shown by (i) increased expression of the recA operon, (ii) excision of the cryptic genetic element e14, (iii) spontaneous induction of lambda, and (iv) dependence of e14 excision on recA+ function. This induction was contingent upon the replication of the P1 Km miniplasmids from their P1 origin and, thus, was apparently caused by an aberrant initiation of DNA replication. When P1 EcoRI-6 was present in cis or trans with a P1 Km miniplasmid, neither e14 nor lambda was excised, but the expression of the recA operon was still induced. These results suggest that P1 EcoRI fragments 5 and 8 are insufficient for normal replication, and thus our P1 Km miniplasmids induced SOS functions. A product of EcoRI-6 may partially restore normal replication.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda/genetics,radiation effects Coliphages/genetics DNA Repair DNA Replication DNA, Bacterial/analysis DNA, Viral/analysis Escherichia coli/genetics Gene Expression Regulation Plasmids Rec A Recombinases Ultraviolet Rays Virus Activation
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Viral Rec A Recombinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Capage M A
Scott J R
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.2.473-480.1983
Published
1983-08-00
Pages
473-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217712
Subset
IM
Grants
NIAID NIH HHS · AI17696 · United States
External Links
PubMed source DOI: 10.1128/jb.155.2.473-480.1983 PMC Full Text View journal details
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