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PMID: 6226649 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 970-4

Baker TA, Howe MM, Gross CA

Abstract

We describe defective Mu phage Mu dX (Mu d1 Bx::Tn9 [lac Apr Cmr]) which is useful for insertion mutagenesis and for construction of lac operon fusions in vivo. Mu dX retains the insertion properties of Mu d1 but produces temperature-resistant lysogens and transposes at a reduced frequency. A method is described to convert existing Mu d1 insertions to Mu dX.

MeSH Terms
Bacteriophage mu/genetics Escherichia coli/genetics Genotype Lac Operon Lysogeny Mutation Species Specificity Temperature Transduction, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baker T A
Howe M M
Gross C A
References (16)
16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.2.970-974.1983
Published
1983-11-00
Pages
970-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217929
Subset
IM
Grants
NIAID NIH HHS · AI 12731 · United States
NIAID NIH HHS · AI-00274 · United States
NIGMS NIH HHS · GM28575 · United States
External Links
PubMed source DOI: 10.1128/jb.156.2.970-974.1983 PMC Full Text View journal details
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