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PMID: 6230373 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ontogeny of Fc receptors and complement receptor (CR3) during human myeloid differentiation.

The Journal of clinical investigation ·Vol. 73 ·No. 2 ·1984-02-00 ·Pages 516-25

Fleit HB, Wright SD, Durie CJ, Valinsky JE, Unkeless JC

Abstract

Two different Fc receptors for IgG (Fc gamma R) have been identified on human leukocytes: a high avidity receptor (Fc gamma Rhi) present on monocytes but not on neutrophils, and a low avidity receptor (Fc gamma Rlo) present on neutrophils but not on monocytes. Fc gamma Rlo can be inhibited and the receptor precipitated by monoclonal antibody 3G8. We have used this monoclonal antibody to study the course of Fc gamma Rlo appearance on bone marrow cells, leukocytes of patients with chronic myelogenous leukemia (CML), and HL-60 and U937 cells induced to differentiate with agents such as dimethyl sulfoxide (DMSO), retinoic acid, phorbol myristate acetate, and lymphokine. We report that Fc gamma Rlo is a late differentiation antigen, first expressed at the metamyelocyte stage. Since precursors to metamyelocytes bear Fc gamma R, and the promyelocyte line HL-60 bears Fc gamma Rhi, there must be a progressive loss of Fc gamma Rhi during myeloid differentiation and the reciprocal expression of Fc gamma Rlo. Results of immunoprecipitation and polyacrylamide gel analysis of the proteins are consistent with these results. We have also studied the receptor for the C3bi complement component (CR3), which is blocked and immunoprecipitated by monoclonal antibody OKM10. During DMSO-driven differentiation of HL-60 cells, we find that CR3 is induced on all cells, whereas Fc gamma Rlo is induced on only 24% of cells, suggesting that CR3 appears earlier during differentiation than Fc gamma Rlo does.

MeSH Terms
Antibodies, Monoclonal Bone Marrow Cells Cell Differentiation Cell Line Granulocytes/cytology,immunology Humans Immunologic Techniques Leukemia, Myeloid/pathology Monocytes/cytology,immunology Receptors, Complement/metabolism Receptors, Complement 3b Receptors, Fc/metabolism
Chemicals
Antibodies, Monoclonal Receptors, Complement Receptors, Complement 3b Receptors, Fc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fleit H B
Wright S D
Durie C J
Valinsky J E
Unkeless J C
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38 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI111238
Published
1984-02-00
Pages
516-25
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC425043
Subset
IM
Grants
NIAID NIH HHS · AI-14603 · United States
NCI NIH HHS · CA-0633 · United States
NCI NIH HHS · CA-30198 · United States
External Links
PubMed source DOI: 10.1172/JCI111238 PMC Full Text View journal details
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