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PMID: 6230414 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isotype specificity of helper T cell clones. Peyer's patch Th cells preferentially collaborate with mature IgA B cells for IgA responses.

The Journal of experimental medicine ·Vol. 159 ·No. 3 ·1984-03-01 ·Pages 798-811

Kiyono H, Cooper MD, Kearney JF, Mosteller LM, Michalek SM, Koopman WJ, McGhee JR

Abstract

The nature of the IgA B cell precursors that receive preferential help from selected clones of T helper cells from mouse Peyer's patches (PP Th A) were studied. Activation of the PP Th A clones required the presence of antigen, sheep erythrocytes (SRBC), in a culture system supporting development of antibody-secreting plasma cells. Two types of PP Th A cells were used. Both gave vigorous IgA responses; the first also supported low IgM, and the second low IgM and IgG subclass antibody responses. Removal of sIgA+ B cells from either splenic or PP B cell cultures selectively depleted precursors of IgA antibody producers. Cultures of purified sIgA+ B cells, cloned PP Th A cells and SRBC, selectively yielded IgA antibody producers. Finally, PP Th A cells did not support IgA responses in B cell cultures derived from spleens of young mice (days 1-25), and full IgA responses were not seen until the donor mice were 6-7 wk of age. These results suggest that cloned T helper cells can recognize and collaborate with mature, IgA committed B cells.

MeSH Terms
Animals Antibody-Producing Cells/classification,cytology,immunology B-Lymphocytes/classification,cytology,immunology Cell Differentiation Clone Cells/immunology Immunoglobulin A/biosynthesis,immunology Immunoglobulin Allotypes/immunology Lymphocyte Cooperation Mice Mice, Inbred C3H Peyer's Patches/cytology Receptors, Antigen, B-Cell/immunology Spleen/cytology T-Lymphocytes, Helper-Inducer/immunology
Chemicals
Immunoglobulin A Immunoglobulin Allotypes Receptors, Antigen, B-Cell
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kiyono H
Cooper M D
Kearney J F
Mosteller L M
Michalek S M
Koopman W J
McGhee J R
References (54)
54 references, click to expand
  1. The carrier effect in the secondary response to hapten-protein conjugates. II. Cellular cooperation.
    Eur J Immunol. 1971 Jan;1(1):18-27 PMID: 14978857
  2. T cell-dependent activation of resting B cells: requirement for both nonspecific unrestricted and antigen-specific Ia-restricted soluble factors.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2497-501 PMID: 6787607
  3. Immune responses in vitro. IV. Suppression of primary M, G, and A plaque-forming cell responses in mouse spleen cell cultures by class-specific antibody to mouse immunoglobulins.
    J Exp Med. 1972 Mar 1;135(3):675-97 PMID: 4536706
  4. Suppression of immunoglobulin class synthesis in mice. I. Effects of treatment with antibody to -chain.
    J Exp Med. 1972 Feb 1;135(2):277-97 PMID: 4551216
  5. A two-stage model for development of antibody-producing cells.
    Clin Exp Immunol. 1972 May;11(1):143-9 PMID: 5064586
  6. The regulatory influence of activated T cells on B cell responses to antigen.
    Adv Immunol. 1972;15:1-94 PMID: 4116319
  7. Cell interactions between histoincompatible T and B lymphocytes. II. Failure of physiologic cooperative interactions between T and B lymphocytes from allogeneic donor strains in humoral response to hapten-protein conjugates.
    J Exp Med. 1973 Jun 1;137(6):1405-18 PMID: 4122709
  8. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes.
    J Exp Med. 1973 Nov 1;138(5):1194-212 PMID: 4542806
  9. Candidate for immunoglobulin D present on murine B lymphocytes.
    Nature. 1974 Dec 13;252(5484):600-2 PMID: 4215039
  10. Interleukin-2 dependent culture of cytolytic T cell lines.
    Immunol Rev. 1981;54:81-109 PMID: 7019041
  11. Individual clones of carrier-specific T cells help idiotypically and isotypically heterogeneous anti-hapten B-cell responses.
    Immunology. 1981 Jul;43(3):581-9 PMID: 6166550
  12. Generation of idiotype-specific T cell help through network perturbation.
    J Exp Med. 1981 Apr 1;153(4):924-35 PMID: 6972994
  13. Influence of Igh-linked gene products on the generation of T helper cells in the response to sheep erythrocytes.
    J Exp Med. 1981 May 1;153(5):1225-35 PMID: 6788888
  14. Priming of T helper cells by antigen-activated B cells. B cell-primed Lyt-1+ helper cells are restricted to cooperate with B cells expressing the IgvH phenotype of the priming B cells.
    J Exp Med. 1981 May 1;153(5):1236-45 PMID: 6788889
  15. Cooperative interaction of B lymphocytes with antigen-specific helper T lymphocytes is MHC restricted.
    Nature. 1981 Aug 6;292(5823):547-9 PMID: 6166872
  16. IgA-specific helper activity of T alpha cells in human peripheral blood.
    J Immunol. 1981 Dec;127(6):2612-3 PMID: 6975332
  17. T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
    J Exp Med. 1982 Mar 1;155(3):734-48 PMID: 7038025
  18. Human b-cell differentiation. I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation.
    J Exp Med. 1982 Mar 1;155(3):839-51 PMID: 6801181
  19. T cell regulation of immunoglobulin class expression in the antibody response to trinitrophenyl-ficoll. Evidence for T cell enhancement of the immunoglobulin class switch.
    J Exp Med. 1982 Mar 1;155(3):884-902 PMID: 6977611
  20. In vivo immune response to a T-cell-dependent antigen by cultures of disassociated murine Peyer's patch.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):596-600 PMID: 6979042
  21. Distinct B cell subpopulations differ in their genetic requirements for activation by T helper cells.
    Immunol Rev. 1982;64:137-60 PMID: 6806171
  22. Role of the major histocompatibility complex in T cell activation of B cell subpopulations. A single monoclonal T helper cell population activates different B cell subpopulations by distinct pathways.
    J Exp Med. 1982 Aug 1;156(2):350-60 PMID: 6980253
  23. The production of membrane or secretory forms immunoglobulins is regulated by C-gene-specific signals.
    Nature. 1982 Sep 9;299(5879):173-5 PMID: 6981066
  24. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help.
    J Immunol. 1983 Feb;130(2):671-7 PMID: 6217248
  25. Human T cell hybridomas secreting factors for IgA-specific help, polyclonal B cell activation, and B cell proliferation.
    J Exp Med. 1982 Dec 1;156(6):1860-5 PMID: 6983562
  26. IgG subclass, IgE, and IgA anti-trinitrophenyl antibody production within trinitrophenyl-Ficoll-responsive B cell clones. Evidence in support of three distinct switching pathways.
    J Exp Med. 1983 Jan 1;157(1):69-85 PMID: 6600273
  27. Murine Peyer's patch T cell clones. Characterization of antigen-specific helper T cells for immunoglobulin A responses.
    J Exp Med. 1982 Oct 1;156(4):1115-30 PMID: 6218215
  28. Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. I. T cells derived from Peyer's patches that switch sIgM B cells to sIgA B cells in vitro.
    J Exp Med. 1983 Feb 1;157(2):433-50 PMID: 6185611
  29. Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. II. Terminal differentiation of postswitch sIgA-bearing Peyer's patch B cells.
    J Exp Med. 1983 Sep 1;158(3):649-69 PMID: 6604126
  30. Differentiation of B lineage cells from liver of neonatal mice: generation of immunoglobulin isotype diversity in vitro.
    J Immunol. 1983 Oct;131(4):1693-7 PMID: 6604750
  31. Cellular cooperation during in vivo anti-hapten antibody responses. I. The effect of cell number on the response.
    J Immunol. 1975 Apr;114(4):1394-401 PMID: 1078834
  32. Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny.
    J Exp Med. 1975 Jan 1;141(1):206-15 PMID: 1090697
  33. The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens.
    J Exp Med. 1975 Aug 1;142(2):460-72 PMID: 1079849
  34. The allogeneic bisection of carrier-specific enhancement of monoclonal B-cell responses.
    J Exp Med. 1975 Nov 1;142(5):1165-79 PMID: 811749
  35. T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells.
    J Exp Med. 1976 Aug 1;144(2):330-44 PMID: 1085324
  36. Regulation by the H-2 gene complex of macrophage-lymphoid cell interactions in secondary antibody responses in vitro.
    J Exp Med. 1976 Aug 1;144(2):371-81 PMID: 956724
  37. Evidence for an immunoglobulin-dependent antigen-specific helper T cell.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4582-6 PMID: 73181
  38. Generation of T helper cells in vitro. IV. F1 T helper cells primed with antigen-pulsed parental macrophages are genetically restricted in their antigen-specific helper activity.
    J Immunol. 1978 Feb;120(2):445-51 PMID: 304458
  39. The role of H-2 linked genes in helper T-cell function. II. Isolation on antigen-pulsed macrophages of two separate populations of F1 helper T cells each specific for antigen and one set of parental H-2 products.
    J Exp Med. 1978 Feb 1;147(2):554-70 PMID: 75242
  40. Restricted helper function of F1 hybrid T cells positively selected to heterologous erythrocytes in irradiated parental strain mice. I. Failure to collaborate with B cells of the opposite parental strain not associated with active suppression.
    J Exp Med. 1978 Apr 1;147(4):1142-58 PMID: 306407
  41. Restricted helper function of F1 hybrid T cells positively selected to heterologous erythrocytes in irradiated parental strain mice. II. Evidence for restrictions affecting helper cell induction and T-B collaboration, both mapping to the K-end of the H-2 complex.
    J Exp Med. 1978 Apr 1;147(4):1159-74 PMID: 306408
  42. Sequential expression of immunoglobulin on developing mouse B lymphocytes: a systematic survey that suggests a model for the generation of immunoglobulin isotype diversity.
    J Immunol. 1978 Jun;120(6):2041-9 PMID: 351064
  43. "Panning" for lymphocytes: a method for cell selection.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844-8 PMID: 351618
  44. T-cell regulation of murine IgA synthesis.
    J Exp Med. 1979 Mar 1;149(3):632-43 PMID: 311811
  45. Nature of T-cell macrophage interaction in helper-cell induction in vitro. II. Two stages of T-helper-cell differentiation analyzed in irradiation and allophenic chimeras.
    J Exp Med. 1979 Mar 1;149(3):686-701 PMID: 311813
  46. Ability of antigen-specific helper cells to effect a class-restricted increase in total Ig-secreting cells in spleens after immunization with the antigen.
    J Exp Med. 1979 Sep 19;150(3):517-30 PMID: 90107
  47. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.
    J Exp Med. 1979 Dec 1;150(6):1510-9 PMID: 92524
  48. Cellular and genetic control of antibody responses. VIII. MHC restricted recognition of accessory cells, not B cells, by parent-specific subpopulations of normal F1 T helper cells.
    J Immunol. 1980 Mar;124(3):1079-85 PMID: 6153669
  49. Absence of an antigen-specific helper T cell required for the expression of the T 15 idiotype in mice treated with anti-mu antibody.
    Eur J Immunol. 1980 Feb;10(2):159-63 PMID: 6154584
  50. T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1612-6 PMID: 6966402
  51. Thymoma production of T cell growth factor (Interleukin 2).
    J Immunol. 1980 Dec;125(6):2555-8 PMID: 6968789
  52. Analysis of histocompatibility requirements for proliferative and helper T cell activity. T cell populations depleted of alloreactive cells by negative selection.
    J Exp Med. 1980 Nov 1;152(5):1311-28 PMID: 6159449
  53. Successive switching of antibody isotypes expressed within the lines of a B-cell clone.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5424-8 PMID: 6776537
  54. Further improvements in the plaque technique for detecting single antibody-forming cells.
    Immunology. 1968 Apr;14(4):599-600 PMID: 5653817
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.159.3.798
Published
1984-03-01
Pages
798-811
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187251
Subset
IM
Grants
NIAID NIH HHS · R01 AI014782 · United States
NIAID NIH HHS · AI 18958 · United States
NIAID NIH HHS · AI18745 · United States
NIDCR NIH HHS · DE 04217 · United States
External Links
PubMed source DOI: 10.1084/jem.159.3.798 PMC Full Text View journal details
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