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PMID: 6231528 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8495-508

Dybvig K, Clark CD, Aliperti G, Schlesinger MJ

Abstract

A DNA sequence consisting of the 5-mer AGAGG repeated tandemly 32 times has been detected in a chicken genomic clone and found to be present in about 2000 copies per chicken genome. This sequence was highly susceptible to single-strand specific endonucleases isolated from Aspergillus oryzae (S1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from Neurospora crassa occurred only at a pH below 5.5. Endonucleolytic cutting of the AGAGG sequence by the single-strand specific enzymes required a supercoiled substrate and was independent of ionic strength.

MeSH Terms
Animals Aspergillus oryzae/enzymology Bacteriophage lambda/genetics Base Sequence Chickens Cloning, Molecular DNA DNA, Single-Stranded Endodeoxyribonucleases/metabolism Neurospora crassa/enzymology Nucleic Acid Hybridization Plants/enzymology Substrate Specificity
Chemicals
DNA, Single-Stranded DNA Endodeoxyribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dybvig K
Clark C D
Aliperti G
Schlesinger M J
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20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.23.8495
Published
1983-12-10
Pages
8495-508
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326598
Subset
IM
Grants
NIGMS NIH HHS · GM 28303 · United States
Databases
GENBANK
X00186
External Links
PubMed source DOI: 10.1093/nar/11.23.8495 PMC Full Text View journal details
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