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PMID: 6232135 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of N-linked oligosaccharide trimming does not interfere with surface expression of certain integral membrane proteins.

The EMBO journal ·Vol. 3 ·No. 3 ·1984-03-00 ·Pages 551-6

Burke B, Matlin K, Bause E, Legler G, Peyrieras N, Ploegh H

Abstract

The effects of 1-deoxynojirimycin (dNM) and 1-deoxymannojirimycin (dMM), inhibitors of oligosaccharide trimming glucosidase I and mannosidase I, respectively, on the biosynthesis of vesicular stomatitis virus G protein, influenza virus hemagglutinin, and human class I histocompatibility antigens were investigated. Although the oligosaccharides of these membrane glycoproteins were greatly altered, neither dNM nor dMM interferred with their surface expression, as determined by a variety of assays, including accessibility to proteases and antibodies; neither did these drugs inhibit production of infectious virus particles.

MeSH Terms

1-Deoxynojirimycin Animals Cell Line Cricetinae Dogs Glucosamine/analogs & derivatives,pharmacology Glucosidases/antagonists & inhibitors Glycoproteins/biosynthesis HLA Antigens/biosynthesis Hemagglutinins, Viral/biosynthesis Mannosidases/antagonists & inhibitors Membrane Proteins/biosynthesis Protein Processing, Post-Translational

Chemicals

Glycoproteins HLA Antigens Hemagglutinins, Viral Membrane Proteins 1-Deoxynojirimycin Glucosidases Mannosidases Glucosamine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Burke B

Matlin K

Bause E

Legler G

Peyrieras N

Ploegh H

References (26)

26 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-03-00

Pages

551-6

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557385

Subset

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PubMed source PMC Full Text View journal details

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Inhibition of N-linked oligosaccharide trimming does not interfere with surface expression of certain integral membrane proteins.

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