Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): construction of his-lac gene fusions.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 764-6

Jayaswal RK, Bressan RA, Handa AK

Abstract

The bacteriophage Mu d1(Apr lac cts62 ) obtained from an Escherichia coli double lysogen carrying the defective Mu d1 phage and a Mu-P1 hybrid phage was utilized as a vector for phage mutagenesis in Erwinia carotovora subsp. carotovora. Among ampicillin-resistant transductants. 1.4% were auxotrophs. The synthesis of beta-galactosidase was derepressed upon starvation for histidine in two different his-lac fusion strains.

MeSH Terms

Alcohol Oxidoreductases/biosynthesis Ampicillin/pharmacology Bacteriophage mu/genetics DNA, Recombinant Enzyme Induction Erwinia/drug effects,genetics,metabolism Histidine/biosynthesis Mutation Operon Penicillin Resistance Transduction, Genetic

Chemicals

DNA, Recombinant Histidine Ampicillin Alcohol Oxidoreductases histidinol dehydrogenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jayaswal R K

Bressan R A

Handa A K

References (11)

11 references, click to expand

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Mutational alteration of a nitrogen-fixing bacterium to sensitivity to infection by bacteriophage Mu: isolation of nif mutations of Klebsiella pneumoniae M5al induced by Mu.
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Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.
J Bacteriol. 1981 Jan;145(1):358-68 PMID: 6450749
Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.
J Bacteriol. 1982 Nov;152(2):959-62 PMID: 6752128
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Methods Enzymol. 1983;100:293-308 PMID: 6312261
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J Biol Chem. 1961 Jul;236:2019-26 PMID: 13682989
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.158.2.764-766.1984

Published

1984-05-00

Pages

764-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215502

Subset

External Links

PubMed source DOI: 10.1128/jb.158.2.764-766.1984 PMC Full Text View journal details

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Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): construction of his-lac gene fusions.

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