Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin.

Applied and environmental microbiology ·Vol. 48 ·No. 2 ·1984-08-00 ·Pages 280-4

Stein DC, Kopec LK, Yasbin RE, Young FE

Abstract

A Bacillus subtilis strain, DSM704, was characterized by genetic means, and its production of a human intestinal sucrase inhibitor, 1-deoxynojirimycin, was described. Synthesis of this compound is detected concomitant with the detection of heat-resistant spores. The amount of 1-deoxynojirimycin produced is highly dependent on the carbon source, with growth on substrates metabolized via glycolysis giving the greatest amount of production (up to 1 mg/ml). 1-Deoxynojirimycin appears to be nonmetabolizable by the producing strain in that it cannot serve as a sole carbon or nitrogen source.

MeSH Terms

1-Deoxynojirimycin Anti-Bacterial Agents/biosynthesis Bacillus subtilis/genetics,growth & development Bacteriophages/genetics Culture Media Glucosamine/analogs & derivatives,biosynthesis Kinetics Sucrase/antagonists & inhibitors Transduction, Genetic

Chemicals

Anti-Bacterial Agents Culture Media 1-Deoxynojirimycin Sucrase Glucosamine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Stein D C

Kopec L K

Yasbin R E

Young F E

References (14)

14 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.48.2.280-284.1984

Published

1984-08-00

Pages

280-4

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC241503

Subset

External Links

PubMed source DOI: 10.1128/aem.48.2.280-284.1984 PMC Full Text View journal details

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Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin.

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