Home LiteratureArticle Details
PMID: 6244539 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Properties of a copper-containing cytochrome c1aa3 complex: a terminal oxidase of the extreme thermophile Thermus thermophilus HB8.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 147-51

Fee JA, Choc MG, Findling KL, Lorence R, Yoshida T

Abstract

From the plasma membrane of Thermus thermophilus HB8 we have partially purified a detergent-solubilized complex of cytochromes a and c1 that actively catalyzes the transfer of electrons from ascorbate via a redox dye to oxygen. The complex is composed of two types of polypeptides, with molecular weights of approximately 55,000 and 33,000. Quantitative analysis revealed the presence of heme a, heme c, and copper in a ratio of 2:1:2, with the heme a being present at 10 +/- 1.3 nmol/mg of protein. The heme c was shown to be associated with the molecular weight 33,000 peptide and is suggested to be of the c1 type. The optical and electron paramagnetic resonance properties of this complex were found to be similar to those of eukaryotic cytochrome oxidase, suggesting the following arrangement of chromophores: a magnetically isolated cytochrome c1 and an oxygen-reducing functional unit consisting of two heme a groups and two copper ions associated with one or more larger peptides.

MeSH Terms
Copper/analysis Cytochromes/analysis,metabolism Electron Spin Resonance Spectroscopy Heme/analysis Hot Temperature Iron/analysis Isoelectric Focusing Molecular Weight Oxygen Consumption Thermodynamics Thermus/enzymology
Chemicals
Cytochromes Heme Copper Iron
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fee J A
Choc M G
Findling K L
Lorence R
Yoshida T
References (33)
33 references, click to expand
  1. Magnetic and spectrophotometric investigations of cytochrome b5.
    Eur J Biochem. 1967 Oct;2(3):361-5 PMID: 4294947
  2. Electronic state of heme in cytochrome oxidase. I. Magnetic circular dichroism of the isolated enzyme and its derivatives.
    J Biol Chem. 1976 Dec 25;251(24):7907-19 PMID: 187599
  3. Physical state of cytochrome oxidase. Relationship between membrane formation and ionic strength.
    Biochim Biophys Acta. 1968 May 28;153(4):804-18 PMID: 4298269
  4. Kinetic studies on the interaction of TMPD with cytochrome c and cytochrome c oxidase.
    Arch Biochem Biophys. 1969 Sep;133(2):327-35 PMID: 4309748
  5. Studies of the heme components of cytochrome c oxidase by EPR spectroscopy.
    Biochim Biophys Acta. 1969 Sep 16;189(1):1-24 PMID: 4309791
  6. EPR studies of the cytochrome b-c 1 segment of the mitochondrial electron transfer system.
    Biochem Biophys Res Commun. 1971 Nov;45(4):871-8 PMID: 4330143
  7. The polypeptide composition of cytochrome oxidase from beef heart mitochondria.
    FEBS Lett. 1972 Oct 1;26(1):261-3 PMID: 4344291
  8. Cytochrome c oxidase: some current biochemical and biophysical problems.
    Q Rev Biophys. 1973 Nov;6(4):389-431 PMID: 4364360
  9. Cytochrome c oxidase from bakers' yeast. III. Physical characterization of isolated subunits and chemical evidence for two different classes of polypeptides.
    J Biol Chem. 1975 Jan 25;250(2):752-61 PMID: 163233
  10. Components of cytochrome c oxidase detectable by EPR spectroscopy.
    Biochim Biophys Acta. 1974 Dec 19;368(3):318-38 PMID: 4375490
  11. EPR signals from cytochrome c oxidase.
    Biochim Biophys Acta. 1976 Feb 13;422(2):260-72 PMID: 174742
  12. Stoichiometry of carbon monoxide binding by cytochrome c oxidase.
    J Biol Chem. 1976 May 10;251(9):2861-2 PMID: 177424
  13. The cytochrome bc1 complex of yeast mitochondria. Isolation and partial characterization of the cytochrome bc1 complex and cytochrome b.
    Eur J Biochem. 1976 May 17;65(1):95-105 PMID: 179821
  14. Characterization of a seventh different subunit of beef heart cytochrome c oxidase. Similarities between the beef heart enzyme and that from other species.
    Biochemistry. 1976 Jun 29;15(13):2930-6 PMID: 181053
  15. An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels.
    Anal Biochem. 1976 Sep;75(1):168-76 PMID: 822747
  16. An infrared study of CO binding to heart cytochrome c oxidase and hemoglobin A. Implications re O2 reactions.
    J Biol Chem. 1977 Aug 10;252(15):5498-508 PMID: 195952
  17. Linear electric field effect in electron paramagnetic resonance for two bisimidazole--heme complexes, model compounds for B and H hemichromes of hemoglobin and for cytochrome b5.
    Biochemistry. 1977 Jun 14;16(12):2795-9 PMID: 196621
  18. Purification and some properties of cytochrome c-552 from an extreme thermophile, Thermus thermophilus HB8.
    J Biochem. 1977 Sep;82(3):769-76 PMID: 199583
  19. C-type cytochromes isloated from an extreme thermophile, Thermus thermophilus HB8.
    J Biochem. 1978 Feb;83(2):629-31 PMID: 204627
  20. Cytochrome c oxidase: a synopsis.
    Arch Biochem Biophys. 1978 May;188(1):1-14 PMID: 209738
  21. Electronic state of heme in cytochrome oxidase III. The magnetic susceptibility of beef heart cytochrome oxidase and some of its derivatives from 7-200 K. Direct evidence for an antiferromagnetically coupled Fe (III)/Cu (II) pair.
    J Biol Chem. 1978 Nov 25;253(22):8065-71 PMID: 213427
  22. The magnetic susceptibility of cytochrome oxidase in the 4.2-1.5 K range.
    J Biol Chem. 1978 Nov 25;253(22):8072-3 PMID: 213428
  23. Analysis of electrophoretic behavior of cytochrome c oxidase subunits. Retardation coefficient versus molecular weight in dodecyl sulfate urea gels.
    Biochim Biophys Acta. 1979 Jan 25;576(1):109-17 PMID: 216410
  24. Cytochrome c1 complexes.
    J Biol Chem. 1979 Mar 25;254(6):1845-53 PMID: 33986
  25. A polyprotein precursor to all four cytoplasmically translated subunits of cytochrome c oxidase from Saccharomyces cerevisiae.
    J Biol Chem. 1979 Jul 25;254(14):6763-71 PMID: 221509
  26. Hyperfine structure resolved by 2 to 4 GHz EPR of cytochrome c oxidase.
    J Biol Chem. 1979 Aug 25;254(16):7482-4 PMID: 224030
  27. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  28. Studies on cytochrome oxidase. III. Improved preparation and some properties.
    J Biol Chem. 1961 Jun;236:1680-8 PMID: 13787373
  29. Properties of the copper associated with cytochrome oxidase as studied by paramagnetic resonance spectroscopy.
    J Biol Chem. 1962 Jul;237:2337-46 PMID: 13866633
  30. OXIDATION-REDUCTION OF THE COPPER COMPONENT OF CYTOCHROME OXIDASE. KINETIC STUDIES WITH A RAPID FREEZING TECHNIQUE.
    J Biol Chem. 1964 Apr;239:1221-7 PMID: 14165930
  31. EFFECT OF ALKALI AND BOROHYDRIDE ON CARDIAC CYTOCHROME OXIDASE. FORMATION OF SCHIFF BASE.
    J Biol Chem. 1965 Jan;240:504-13 PMID: 14253462
  32. Prosthetic groups of the cytochromes present in Corynebacterium diphtheriae with especial reference to cytochrome a.
    Biochem J. 1949;45(3):247-55, pl PMID: 15392846
  33. Electron paramagnetic resonance of beef-heart ferricytochrome c.
    J Chem Phys. 1968 Mar 1;48(5):2049-52 PMID: 4297179
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.1.147
Published
1980-01-00
Pages
147-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348225
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.1.147 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2