Cloning of the herpes simplex virus type 1 thymidine kinase gene in E. coli K 12: a selective marker for gene transfer into animal cells.

Developments in biological standardization ·Vol. 46 ·1980-00-00 ·Pages 75-82

Colbere-Garapin F, Horodniceanu F, Kourilsky P, Garapin AC

Abstract

The Herpes Simplex Virus type 1 (HSV 1) thymidine kinase (TK) gene, carried by a 3.6 kb Bam H1 DNA fragment, was isolated and ligated to Bam H1 cleaved bacterial plasmid pBR 322. The TK gene, cloned in E. coli, is functional when it is transferred back into Eukaryotic cells, and can thus be used as a selective marker for gene transfer.

MeSH Terms

Animals Cloning, Molecular Escherichia coli/genetics Genes, Viral Genetic Markers L Cells Mice Plasmids Simplexvirus/genetics Thymidine Kinase/genetics Transfection

Chemicals

Genetic Markers Thymidine Kinase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Colbere-Garapin F

Horodniceanu F

Kourilsky P

Garapin A C

Article Info

Journal

Developments in biological standardization

Abbr.

Dev Biol Stand

ISSN

0301-5149

Published

1980-00-00

Pages

75-82

Language

English

Region

Switzerland

NLM ID

0427140

Subset

External Links

PubMed source

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Cloning of the herpes simplex virus type 1 thymidine kinase gene in E. coli K 12: a selective marker for gene transfer into animal cells.

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