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PMID: 6245236 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

In vitro recombination of bacteriophage T7 DNA damaged by UV radiation.

Journal of virology ·Vol. 33 ·No. 1 ·1980-01-00 ·Pages 330-9

Masker WE, Kuemmerle NB

Abstract

A system capable of in vitro packaging of exogenous bacteriophage T7 DNA has been used to monitor the biological activity of DNA replicated in vitro. This system has been used to follow the effects of UV radiation on in vitro replication and recombination. During the in vitro replication process, a considerable exchange of genetic information occurs between T7 DNA molecules present in the reaction mixture. This in vitro recombination is reflected in the genotype of the T7 phage produced after in vitro encapsulation; depending on the genetic markers selected, recombinants can comprise nearly 20% of the total phage production. When UV-irradiated DNA is incubated in this system, the amount of in vitro synthesis is reduced and the total amount of viable phage produced after in vitro packaging is diminished. In vitro recombination rates are also lower when the participating DNA molecules have been exposed to UV. However, biochemical and genetic measurements confirmed that there is little or no transfer of pyrimidine dimers from irradiated DNA into undamaged molecules.

MeSH Terms
Cell-Free System DNA Replication/radiation effects DNA, Viral/biosynthesis,radiation effects Exonucleases/genetics Genes, Viral Genetic Markers Recombination, Genetic/radiation effects T-Phages/genetics,metabolism,radiation effects Ultraviolet Rays
Chemicals
DNA, Viral Genetic Markers Exonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Masker W E
Kuemmerle N B
References (36)
36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.33.1.330-339.1980
Published
1980-01-00
Pages
330-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288550
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.33.1.330-339.1980 PMC Full Text View journal details
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