Ultraviolet-endonuclease activity in cell extracts of Saccharomyces cerevisiae mutants defective in excision of pyrimidine dimers.

Journal of bacteriology ·Vol. 142 ·No. 1 ·1980-04-00 ·Pages 322-4

Bekker ML, Kaboev OK, Akhmedov AT, Luchkina LA

Abstract

Cell-free extracts of ultraviolet-sensitive mutants of Saccharomyces cerevisiae defective in excision of pyrimidine dimers, rad1, rad2, rad3, rad4, rad10, and rad16, as well as the extracts of the wild-type strain RAD+, display ultraviolet-endonuclease activity.

MeSH Terms

DNA, Fungal/metabolism,radiation effects Endonucleases/metabolism Mutation Pyrimidine Dimers/metabolism Saccharomyces cerevisiae/enzymology,genetics Ultraviolet Rays

Chemicals

DNA, Fungal Pyrimidine Dimers Endonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bekker M L

Kaboev O K

Akhmedov A T

Luchkina L A

References (12)

12 references, click to expand

The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast.
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Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
Enzymatic repair of DNA.
Annu Rev Biochem. 1975;44:19-43 PMID: 1094910
Effect of mutations in lig and polA on UV-induced strand cutting in a uvrC strain of Escherichia coli.
Basic Life Sci. 1975;5B:439-41 PMID: 1103860
Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet light.
J Mol Biol. 1975 Nov 15;98(4):781-95 PMID: 1104879
Defective thymine dimer excision by cell-free extracts of xeroderma pigmentosum cells.
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2757-61 PMID: 1066689
Defective thymine dimer excision in radiation-sensitive mutants rad10 and rad16 of Saccharomyces cerevisiae.
Mol Gen Genet. 1977 Apr 29;152(3):125-8 PMID: 327268
Repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6 and rad9 of Saccharomyces cerevisiae.
Mutat Res. 1977 Oct;45(1):13-20 PMID: 335235
Removal of pyrimidine dimers from Saccharomyces cerevisiae nuclear DNA under nongrowth conditions as detected by a sensitive, enzymatic assay.
Mutat Res. 1978 Apr;50(1):43-56 PMID: 347280
A new method for the purification and identification of covalently closed circular DNA molcules.
Nucleic Acids Res. 1978 Apr;5(4):1139-52 PMID: 26042
Reconstitution of an Escherichia coli repair endonuclease activity from the separated uvrA+ and uvrB+/uvrC+ gene products.
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2569-73 PMID: 351611
Endonuclease alpha from Saccharomyces cerevisiae shows increased activity on ultraviolet irradiated native DNA.
Mol Gen Genet. 1978 Nov 29;167(2):139-45 PMID: 366383

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.142.1.322-324.1980

Published

1980-04-00

Pages

322-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC293959

Subset

External Links

PubMed source DOI: 10.1128/jb.142.1.322-324.1980 PMC Full Text View journal details

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Ultraviolet-endonuclease activity in cell extracts of Saccharomyces cerevisiae mutants defective in excision of pyrimidine dimers.

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