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PMID: 6246274 Published · ppublish English Journal Article

Novel p19-related protein in Rous-associated virus type 61: implications for avian gag gene order.

Journal of virology ·Vol. 34 ·No. 2 ·1980-05-00 ·Pages 431-7

Shealy DJ, Mosser AG, Rueckert RR

Abstract

Virions of Rous-associated virus type 61 contain a previously unrecognized p19-related protein, called p19f, which comigrates with gag protein p12 during electrophoresis in sodium dodecyl sulfate-polyacrylamide gels but can be separated by gel filtration chromatography in 6 M guanidine hydrochloride. It is shown that the existence of p19f accounts for the earlier inability to order p27 and p12 by the pactamycin mapping procedure. Remapping with pactamycin by using methods which take this new protein into account yielded a gag gene order of NH2-p219-p27-p12-p15-COOH. It also confirmed earlier positions for the env and pol genes and placed unclassified protein p10 near a translational initiation site. The pactamycin-derived mapping position of p12 differs from reports based on tryptic analysis. An analysis of procedural shortcomings emphasizes the need for more definitive determinations of the avian gag gene order.

MeSH Terms
Avian Leukosis Virus/analysis,genetics Chromatography, Gel Electrophoresis, Polyacrylamide Gel Genes, Viral Pactamycin/pharmacology Protein Biosynthesis Viral Proteins/analysis,genetics
Chemicals
Viral Proteins Pactamycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shealy D J
Mosser A G
Rueckert R R
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38 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.2.431-437.1980
Published
1980-05-00
Pages
431-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288721
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.34.2.431-437.1980 PMC Full Text View journal details
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