Abstract
We have prepared (125)I-labeled cholecystokinin and have examined the kinetics, stoichiometry, and chemical specificity with which the labeled peptide binds to dispersed acini from guinea pig pancreas. Binding of (125)I-labeled cholecystokinin was reversible, temperature-dependent, saturable, specific, and localized to the plasma membrane. Each acinar cell possessed approximately 9000 binding sites, and binding of the labeled peptide to these sites could be inhibited by cholecystokinin and structurally related peptides (e.g., gastrin and caerulein) as well as by nonpeptide competitive antagonists of the action of cholecystokinin. Binding was not inhibited by other pancreatic secretagogues such as secretin, vasoactive intestinal peptide, glucagon, physalaemin, eledoisin, kassinin, substance P, carbamoylcholine, litorin, or ranatensin or by bovine pancreatic polypeptide, atropine, neurotensin, leucineenkephalin, methionine-enkephalin, or cyclic somatostatin. With agonists as well as antagonists there was a good correlation between occupation of cholecystokinin binding sites and changes in acinar cell function. With each of six different peptide agonists maximal stimulation of enzyme secretion occurred with 40% receptor occupation and occupation of the remaining 60% caused a progressive decrease in stimulated amylase release. Agonists, but not antagonists, accelerated the dissociation of bound (125)I-labeled cholecystokinin, and these findings suggest that, in pancreatic acini, radiolabeled cholecystokinin binds to at least one class of interacting binding sites whose affinities are influenced by the extent to which these sites are occupied by agonists but not the extent to which they are occupied by antagonists.
MeSH Terms
Animals
Cell Membrane/metabolism
Cholecystokinin/metabolism
Cyclic GMP/metabolism
Dibutyryl Cyclic GMP/metabolism
Guinea Pigs
Kinetics
Pancreas/metabolism
Peptide Fragments/metabolism
Receptors, Cell Surface/metabolism
Structure-Activity Relationship
Temperature
Chemicals
Peptide Fragments
Receptors, Cell Surface
Dibutyryl Cyclic GMP
Cholecystokinin
Cyclic GMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jensen R T
Lemp G F
Gardner J D
References (19)
19 references, click to expand
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