The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

The Biochemical journal ·Vol. 185 ·No. 1 ·1980-01-01 ·Pages 59-63

Hughes SG, Bruce T, Murray K

Abstract

An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments.

MeSH Terms

Bacteriophage lambda Base Sequence Cyanobacteria/enzymology DNA, Viral Electrophoresis, Agar Gel Endonucleases/isolation & purification Oligodeoxyribonucleotides/analysis

Chemicals

DNA, Viral Oligodeoxyribonucleotides Endonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hughes S G

Bruce T

Murray K

References (15)

15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1850059

Published

1980-01-01

Pages

59-63

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161269

Subset

External Links

PubMed source DOI: 10.1042/bj1850059 PMC Full Text View journal details

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The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.

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