Biological activities of fragments derived from Bordetella pertussis endotoxin: isolation of a nontoxic, Shwartzman-negative lipid A possessing high adjuvant properties.

Infection and immunity ·Vol. 27 ·No. 3 ·1980-03-00 ·Pages 739-45

Ayme G, Caroff M, Chaby R, Haeffner-Cavaillon N, Le Dur A, Moreau M, Muset M, Mynard MC, Roumiantzeff M, Schulz D, Szabó L

Abstract

Endotoxin from fresly sedimented Bordetella pertussis cells, isolated by the phenol/water procedure when submitted to kinetically controlled, mild acidic hydrolysis released a polysaccharide (polysaccharide 1), a complex lipid (lipid X), and a glycolipid. When treated with somewhat stronger acid, the glycolipid yielded a second polysaccharide (polysaccharide 2) and another complex lipid (lipid A). The intact pertussis endotoxin had all the usual properties of endotoxins extracted from enteric bacteria. Lipid X and the intermediary glycolipid retained all the endotoxic properties of the unfractionated endotoxin. In lipid A, pyrogenicity was reduced to a very low level and toxicity and Shwartzman reactivity were absent; however, this fraction retained most of the endotoxin's antiviral activity, and its adjuvant power was considerably higher than that of the intact endotoxin. Lipid A elicited nonspecific resistance against challenge with certain bacteria, but not against others.

MeSH Terms

Adjuvants, Immunologic Bacterial Toxins/analysis Bordetella pertussis Endotoxins/analysis Fever/chemically induced Lipid A/immunology,isolation & purification,pharmacology Lipopolysaccharides/pharmacology Shwartzman Phenomenon Viruses/drug effects

Chemicals

Adjuvants, Immunologic Bacterial Toxins Endotoxins Lipid A Lipopolysaccharides

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Ayme G

Caroff M

Chaby R

Haeffner-Cavaillon N

Le Dur A

Moreau M

Muset M

Mynard M C

Roumiantzeff M

Schulz D

Szabó L

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.27.3.739-745.1980

Published

1980-03-00

Pages

739-45

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC550835

Subset

External Links

PubMed source DOI: 10.1128/iai.27.3.739-745.1980 PMC Full Text View journal details

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Biological activities of fragments derived from Bordetella pertussis endotoxin: isolation of a nontoxic, Shwartzman-negative lipid A possessing high adjuvant properties.

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