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PMID: 6247620 Published · ppublish English Journal Article

Construction of a physical map of a kanamycin (Km) transposon, Tn5, and a comparison to another Km transposon, Tn903.

Molecular & general genetics : MGG ·Vol. 178 ·No. 1 ·1980-04-00 ·Pages 77-83

Yamamoto T, Yokota T

Abstract

A cleavage map of Tn5, a kanamycin (Km) transposon from plasmid JR67, was constructed from pMKI, a composite plasmid of ColE1 and Tn5, and compared to that of Tn903, a Km transposon from plasmid R6-5. The two transposons showed marked heterogeneity in both the structural gene for Km resistance and the inverted repeat regions as evidenced by their distinctly different restriction maps. This result suggests separate paths of evolution for the two Km transposons.

MeSH Terms
Base Sequence Chromosome Inversion Chromosome Mapping DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/analysis,genetics Drug Resistance, Microbial Genes Kanamycin/pharmacology Phenotype
Chemicals
DNA Transposable Elements DNA, Bacterial Kanamycin DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yamamoto T
Yokota T
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24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267215
Published
1980-04-00
Pages
77-83
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267215
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