Molecular cloning of EcoRII endonuclease and methylase genes.

Molecular & general genetics : MGG ·Vol. 178 ·No. 3 ·1980-00-00 ·Pages 717-8

Kosykh VG, Buryanov YI, Bayev AA

Abstract

The genes for restriction-modification system EcoRII have been cloned from plasmid N3 DNA using RSF2124 as a vector plasmid. The hybrid plasmids designated pFK321 and pFK322 contained a 5.8 megadaltons EcoRI--fragment derived from N3 DNA including the genes for restriction-modification system EcoRII and a gene for resistance to sulfanilamide.

MeSH Terms

Bacteriophage lambda/genetics Cloning, Molecular DNA Restriction Enzymes/genetics Escherichia coli/genetics Genes Genes, Viral Methyltransferases/genetics Plasmids Sulfanilamides/pharmacology

Chemicals

Sulfanilamides Methyltransferases DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kosykh V G

Buryanov Y I

Bayev A A

References (8)

8 references, click to expand

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The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.
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Dokl Akad Nauk SSSR. 1978;238(5):1227-30 PMID: 344017
A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3900-4 PMID: 1069275
Chromosome replication and the division cycle of Escherichia coli B/r.
J Mol Biol. 1968 Feb 14;31(3):519-40 PMID: 4866337
Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
Location of the region controlling host specificity (hsII) with respect to drug resistance markers on the fi - R factor, N-3.
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J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00337884

Published

1980-00-00

Pages

717-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00337884

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Molecular cloning of EcoRII endonuclease and methylase genes.

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