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PMID: 6248880 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a proviral genome associated with avian myeloblastic leukemia.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 3004-8

Souza LM, Komaromy MC, Baluda MA

Abstract

We have identified and isolated a presumptive leukemogenic provirus from myeloblasts of a chicken in which leukemia had been induced by avian myeloblastosis virus (AMV). Leukemic myeloblasts isolated from peripheral blood or from converted yolk sac cultures of various strains of chickens, regardless of the endogenous proviral content or AMV pseudotype used for infection, contain an EcoRI 2.2-megadalton (MDaI) and a HindIII 2.6-MDal proviral fragment. A proviral genome flanked by chicken DNA sequences on either side and containing both the EcoRI 2.2-MDal and the HindIII 2.6-MDal fragments was inserted by molecular recombination into lambda phage Charon 4A and then cloned. This presumptive AMV proviral genome has a mass of approximately 4.9 MDal and contains terminal redundancies with respect to 3' viral RNA sequences.

MeSH Terms
Animals Avian Leukosis/microbiology Avian Leukosis Virus/genetics Avian Myeloblastosis Virus/genetics Cells, Cultured Chickens DNA Restriction Enzymes DNA, Recombinant Defective Viruses/isolation & purification Genes, Viral Helper Viruses/genetics Nucleic Acid Hybridization RNA, Viral/genetics
Chemicals
DNA, Recombinant RNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Souza L M
Komaromy M C
Baluda M A
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.3004
Published
1980-05-00
Pages
3004-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349535
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.3004 PMC Full Text View journal details
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