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PMID: 6252189 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synchronization of cell division in microorganisms by percoll gradients.

Journal of bacteriology ·Vol. 144 ·No. 1 ·1980-10-00 ·Pages 17-21

Dwek RD, Kobrin LH, Grossman N, Ron EZ

Abstract

We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.

MeSH Terms

Bacillus subtilis/cytology Cell Division Centrifugation, Density Gradient Colloids Cytological Techniques Escherichia coli/cytology Microbiological Techniques Povidone Schizosaccharomyces/cytology Silicon Dioxide

Chemicals

Colloids Silicon Dioxide Povidone

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Dwek R D

Kobrin L H

Grossman N

Ron E Z

References (12)

12 references, click to expand

Synchronization of cell division in Escherichia coli by amino acid starvation: strain specificity.
J Bacteriol. 1975 Jul;123(1):374-6 PMID: 1095561
Fluctuations in buoyant density during the cell cycle of Escherichia coli K12: significance for the preparation of synchronous cultures by age selection.
J Gen Microbiol. 1977 Jan;98(1):177-86 PMID: 319198
Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth.
J Bacteriol. 1977 Jul;131(1):270-9 PMID: 326763
Physical methods for obtaining synchronous culture of Escherichia coli.
J Bacteriol. 1956 May;71(5):542-6 PMID: 13331866
Control of cell division in Escherichia coli: effect of amino acid starvation.
J Bacteriol. 1977 Feb;129(2):569-73 PMID: 320184
The viability of cells grown or centrifuged in a new density gradient medium, Percoll(TM).
Exp Cell Res. 1977 Dec;110(2):449-57 PMID: 201479
Quantitative assessment of the synchronization of cell populations.
J Gen Microbiol. 1979 Jan;110(1):225-7 PMID: 372491
Synchronous growth of enteric bacteria.
J Bacteriol. 1970 Sep;103(3):789-92 PMID: 4919993
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
COMPARISON OF SYNCHRONOUS AND SYNCHRONIZED CELL DIVISION.
Exp Cell Res. 1964 Jan;33:89-98 PMID: 14109157
The induction of cellular and nuclear division in Salmonella typhimurium by means of temperature shifts.
Biochim Biophys Acta. 1954 Nov;15(3):345-56 PMID: 13230073
Deoxyribonucleic acid synthesis during the division cycle of Escherichia coli: a comparison of strains B-r, K-12, 15, and 15T- under conditions of slow growth.
J Bacteriol. 1974 Mar;117(3):1216-23 PMID: 4591948

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.144.1.17-21.1980

Published

1980-10-00

Pages

17-21

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC294577

Subset

External Links

PubMed source DOI: 10.1128/jb.144.1.17-21.1980 PMC Full Text View journal details

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