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PMID: 6252211 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transport of the membrane glycoprotein of vesicular stomatitis virus to the cell surface in two stages by clathrin-coated vesicles.

The Journal of cell biology ·Vol. 86 ·No. 1 ·1980-07-00 ·Pages 162-71

Rothman JE, Bursztyn-Pettegrew H, Fine RE

Abstract

The G protein of vesicular stomatitis virus is a transmembrane glycoprotein that is transported from its site of synthesis in the rough endoplasmic reticulum to the plasma membrane via the Golgi apparatus. Pulse-chase experiments suggest that G is transported to the cell surface in two successive waves of clathrin-coated vesicles. The oligosaccharides of G protein carried in the early wave are of the "high-mannose" (G1) form, whereas the oligosaccharides in the second, later wave are of the mature "complex" (G2) form. the early wave is therefore proposed to correspond to transport of G in coated vesicles from the endoplasmic reticulum to the Golgi apparatus, and the succeeding wave to transport from the Golgi apparatus to the plasma membrane. The G1- and G2-containing coated vesicles appear to be structurally distinct, as judged by their differential precipitation by anticoated vesicle serum.

MeSH Terms
Animals Biological Transport, Active Cell Compartmentation Clathrin Cricetinae Cytoplasmic Granules/metabolism Endoplasmic Reticulum/metabolism Glycoproteins/metabolism Golgi Apparatus/metabolism Intracellular Membranes/metabolism Membrane Proteins/metabolism Vesicular stomatitis Indiana virus Viral Proteins/biosynthesis,metabolism
Chemicals
Clathrin Glycoproteins Membrane Proteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rothman J E
Bursztyn-Pettegrew H
Fine R E
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31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.86.1.162
Published
1980-07-00
Pages
162-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110647
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.86.1.162 PMC Full Text View journal details
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