Home LiteratureArticle Details
PMID: 6253621 Published · ppublish English Journal Article

The action of serotonin in the rat hippocampal slice preparation.

The Journal of physiology ·Vol. 303 ·1980-06-00 ·Pages 423-39

Segal M

Abstract

1. Intracellular activity was recorded from neurones in the CA1 pyramidal layer of slices of rat hippocampus maintained in vitro. 2. Application of 5-HT in a droplet or via ionophoresis produced a 3-5 mV hyperpolarization associated with a 30% decrease in input resistance. 3. The response to 5-HT was minimal with a drop concentration of 1 microM and maximal with 100 microM. The responses appeared to be blocked by methysergide applied in the superfusion medium. 4. The responses to 5-HT were minimal when the drug was applied in the apical dendritic region and maximal when it was applied near the soma. 5. 5-HT produced no substantial changes in e.p.s.p.s evoked by stimulation of the Schaffer collateral-commissural system or in i.p.s.p.s which were occasionally encountered following stimuli to the stratum radiatum. 6. The responses to 5-HT are true post-synaptic responses and are not indirect effects since they are present in a Ca2+-deficient Mg2+-enriched medium which blocks synaptic transmission. 7. The responses to 5-HT were not dependent on extracellular Cl- concentration. 8. These experiments indicate that 5-HT produces its effects in the rat hippocampus by activating K+ channels.

MeSH Terms
Animals Electric Conductivity Hippocampus/drug effects,physiology In Vitro Techniques Ion Channels/drug effects Male Membrane Potentials/drug effects Neurons/drug effects Potassium/metabolism Rats Serotonin/pharmacology
Chemicals
Ion Channels Serotonin Potassium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Segal M
References (29)
29 references, click to expand
  1. The inhibitory action of monoamines on lateral geniculate neurones.
    J Physiol. 1967 Jun;190(3):563-81 PMID: 6051787
  2. Connections of the median and dorsal raphe nuclei in the rat: an autoradiographic and degeneration study.
    J Comp Neurol. 1974 Jul;156(2):179-205 PMID: 4419253
  3. Further studies on the mode of action of psychotomimetic drugs: antagonism of the excitatory actions of 5-hydroxytryptamine by methylated derivatives of tryptamine.
    Br J Pharmacol. 1974 Mar;50(3):345-54 PMID: 4277490
  4. Generation of slow postsynaptic potentials without increases in ionic conductance.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1304-11 PMID: 4299944
  5. The modification of lumbar motoneurone excitability by stimulation of a putative 5-hydroxytryptamine pathway.
    Br J Pharmacol. 1974 Nov;52(3):339-48 PMID: 4458843
  6. Hippocampal innervation by serotonin neurons of the midbrain raphe in the rat.
    J Comp Neurol. 1975 Nov 15;164(2):171-83 PMID: 52660
  7. Characterization of the monoaminergic innervation of immature rat neocortex: a histofluorescence analysis.
    J Comp Neurol. 1978 Oct 1;181(3):663-79 PMID: 357458
  8. Endogenous norepinephrine and serotonin within the hippocampal formation during the development and recovery from septal hyperreactivity.
    Pharmacol Biochem Behav. 1978 Sep;9(3):359-67 PMID: 714984
  9. Functional characteristics of unmyelinated fibres in the hippocampal cortex.
    Brain Res. 1978 Apr 7;144(1):11-8 PMID: 638755
  10. Effects of serotonin on central neurons: microiontophoretic administration.
    Fed Proc. 1972 Jan-Feb;31(1):97-106 PMID: 4333253
  11. Serotonin effects on central neurons.
    Adv Pharmacol. 1968;6(Pt A):395-413 PMID: 4298808
  12. A central inhibitory action of 5-hydroxytryptamine in the leech.
    J Neurobiol. 1976 Nov;7(6):477-82 PMID: 1003196
  13. Afferents to the hippocampus of the rat studied with the method of retrograde transport of horseradish peroxidase.
    Brain Res. 1974 Sep 20;78(1):1-15 PMID: 4458909
  14. Inhibiton of neurons in the amygdala by dorsal raphe stimulation: mediation through a direct serotonergic pathway.
    Brain Res. 1977 Jan 14;120(1):85-102 PMID: 137766
  15. Voltage-dependent calcium current induced by serotonin.
    Nature. 1979 Feb 8;277(5696):483-4 PMID: 763332
  16. Synaptic connexions of two symmetrically placed giant serotonin-containing neurones.
    J Physiol. 1974 Jan;236(2):435-64 PMID: 16992445
  17. Activation of hippocampal neurons by mossy fiber stimulation in thin brain sections in vitro.
    Exp Brain Res. 1972;14(4):423-35 PMID: 4340851
  18. Characteristics of CA1 neurons recorded intracellularly in the hippocampal in vitro slice preparation.
    Brain Res. 1975 Mar 7;85(3):423-36 PMID: 1111846
  19. Inhibition of cerebral cortical neurones by a 5-hydroxytryptaminergic pathway from median raphé nucleus.
    Can J Physiol Pharmacol. 1977 Jun;55(3):737-43 PMID: 884623
  20. Physiological and pharmacological evidence for a serotonergic projection to the hippocampus.
    Brain Res. 1975 Aug 22;94(1):115-131 PMID: 167913
  21. An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat.
    J Comp Neurol. 1978 Jun 1;179(3):641-67 PMID: 565370
  22. Unit analysis of hippocampal polulation spikes.
    Exp Brain Res. 1971;13(2):208-21 PMID: 5123965
  23. Ionic mechanisms and receptor properties underlying the responses of molluscan neurones to 5-hydroxytryptamine.
    J Physiol. 1974 Dec;243(2):427-56 PMID: 4155767
  24. The effects of brainstem priming stimulation on interhemispheric hippocampal responses in the awake rat.
    Exp Brain Res. 1977 Jul 15;28(5):529-41 PMID: 196875
  25. Serotonin and lysergic acid diethylamide binding in rat brain membranes: relationship to postsynaptic serotonin receptors.
    Mol Pharmacol. 1976 May;12(3):373-89 PMID: 6896
  26. Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat.
    Mol Pharmacol. 1978 Nov;14(6):983-95 PMID: 732817
  27. 5-HT antagonists in rat hippocampus.
    Brain Res. 1976 Feb 13;103(1):161-6 PMID: 3262
  28. Further evidence for a possible excitatory serotonergic synapse on raphe neurons of pons and lower midbrain.
    Life Sci. 1976 Sep 1;19(5):761-7 PMID: 957906
  29. Excitation and depression of cortical neurones by 5-hydroxytryptamine.
    J Physiol. 1967 Nov;193(2):269-94 PMID: 6065878
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1980.sp013297
Published
1980-06-00
Pages
423-39
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1282903
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1980.sp013297 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2