Home LiteratureArticle Details
PMID: 6254007 Published · ppublish English Journal Article

Cytoplasmically made subunits of yeast mitochondrial F1-ATPase and cytochrome c oxidase are synthesized as individual precursors, not as polyproteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 3998-4002

Lewin AS, Gregor I, Mason TL, Nelson N, Schatz G

Abstract

At least three subunits of yeast mitochondrial F1-ATPase (ATP phosphohydrolase, EC 3.6.1.3) and at least two subunits of cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) are synthesized outside the mitochondria and imported into the organelles as individual precursors that are between 2000 and 6000 daltons larger than the mature subunits. These precursors were shown to be primary translation products. Therefore, neither the five F1 subunits nor the four small cytochrome c oxidase subunits are synthesized as a single polyprotein.

MeSH Terms
Adenosine Triphosphatases/biosynthesis Cytoplasm/metabolism Electron Transport Complex IV/biosynthesis Intracellular Membranes/enzymology Macromolecular Substances Membrane Proteins/biosynthesis Mitochondria/enzymology Peptide Fragments/metabolism Protein Precursors/metabolism Saccharomyces cerevisiae/metabolism
Chemicals
Macromolecular Substances Membrane Proteins Peptide Fragments Protein Precursors Electron Transport Complex IV Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lewin A S
Gregor I
Mason T L
Nelson N
Schatz G
References (21)
21 references, click to expand
  1. [Fractionation of Brewer's yeast t-RNA by countercurrent distribution].
    Bull Soc Chim Biol (Paris). 1967;49(12):1679-87 PMID: 5582428
  2. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.
    Anal Biochem. 1971 Feb;39(2):462-77 PMID: 4101989
  3. Initiation of hemoglobin synthesis. Specific inhibition by antibiotics and bacteriophage ribonucleic acid.
    Biochemistry. 1971 Jun 8;10(12):2348-56 PMID: 5114993
  4. Preparation and analysis of L-( 35 S)methionine labeled transfer ribonucleic acids from rabbit liver.
    Anal Biochem. 1972 Jul;48(1):202-16 PMID: 4557421
  5. Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. II. Effect of nuclear and extrachromosomal mutations on the formation of cytochrome c oxidase.
    J Biol Chem. 1973 Aug 10;248(15):5369-78 PMID: 4358613
  6. Cytochrome c oxidase from bakers' yeast. IV. Immunological evidence for the participation of a mitochondrially synthesized subunit in enzymatic activity.
    J Biol Chem. 1975 Jan 25;250(2):762-6 PMID: 163234
  7. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  8. Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1083-6 PMID: 772678
  9. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  10. Regulation of mitochondrial protein synthesis by cytoplasmic proteins.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3947-51 PMID: 186780
  11. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  12. Precursor of egg white lysozyme. Amino acid sequence of an NH2-terminal extension.
    J Biol Chem. 1977 Sep 25;252(18):6386-93 PMID: 893412
  13. Kinetic studies on the transport of cytoplasmically synthesized proteins into the mitochondria in intact cells of Neurospora crassa.
    Eur J Biochem. 1977 Dec;81(3):523-32 PMID: 202461
  14. Biogenesis of the cytochrome bc1 complex of yeast mitochondria. A precursor form of the cytoplasmically made subunit V.
    J Biol Chem. 1979 Mar 10;254(5):1437-9 PMID: 216693
  15. Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):343-7 PMID: 154672
  16. A polyprotein precursor to all four cytoplasmically translated subunits of cytochrome c oxidase from Saccharomyces cerevisiae.
    J Biol Chem. 1979 Jul 25;254(14):6763-71 PMID: 221509
  17. Post-translational processing and transport of the polyprotein precursor to subunits IV to VII of yeast cytochrome c oxidase.
    J Biol Chem. 1979 Jul 25;254(14):6772-80 PMID: 221510
  18. Transport of proteins across the mitochondrial outer membrane. A precursor form of the cytoplasmically made intermembrane enzyme cytochrome c peroxidase.
    J Biol Chem. 1979 Aug 25;254(16):7468-71 PMID: 224028
  19. The precursor for carbamyl phosphate synthetase is transported to mitochondria via a cytosolic route.
    J Biol Chem. 1979 Oct 10;254(19):9335-8 PMID: 226513
  20. Energy-dependent processing of cytoplasmically made precursors to mitochondrial proteins.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4365-9 PMID: 388440
  21. Cell-free synthesis of cytochrome c.
    FEBS Lett. 1979 Dec 1;108(1):141-6 PMID: 230077
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.7.3998
Published
1980-07-00
Pages
3998-4002
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349755
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.7.3998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2