Genomic organization of rat prolactin and growth hormone genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 8 ·1980-08-00 ·Pages 4583-7

Chien YH, Thompson EB

Abstract

Five overlapping cloned DNAs containing the rat prolactin gene and its flanking sequences, as well as one cloned DNA containing the rat growth hormone gene and its flanking sequences, were isolated from a chromosomal DNA library. They were characterized by restriction enzyme mapping and electron microscopy. In each gene, the structural gene sequence coding for mature mRNA of a length of about 1 kilobase is split into at least five segments by a minimum of four intervening sequences. The two genes are similar in the length and organization of their coding regions, consistent with the suggestion that they are derived from a common ancestral gene. However, the two genes differ greatly in the lengths of their intervening sequences. That leads to a total gene length of 10 kilobase pairs for the prolactin and 2.1 kilobase pairs for the growth hormone gene. At least one intervening sequence appears to be in the 5' nontranslated regions of the prolactin and growth hormone mRNA coding sequences.

MeSH Terms

Animals Base Sequence DNA Restriction Enzymes DNA, Recombinant Female Genes Growth Hormone/genetics Nucleic Acid Hybridization Pituitary Gland/metabolism Prolactin/genetics RNA, Messenger/genetics Rats Transcription, Genetic

Chemicals

DNA, Recombinant RNA, Messenger Prolactin Growth Hormone DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Chien Y H

Thompson E B

References (24)

24 references, click to expand

Polyadenylic acid-containing RNA in Xenopus laevis oocytes.
J Mol Biol. 1974 May 5;85(1):87-101 PMID: 4835729
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
Thyrotropin-releasing hormone increases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free system.
Biochem Biophys Res Commun. 1976 Jun 21;70(4):1180-9 PMID: 821486
Mapping of poly(A) sequences in the electron microscope reveals unusual structure of type C oncornavirus RNA molecules.
Cell. 1976 Apr;7(4):595-607 PMID: 182376
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
Triiodothyronine stimulates specifically growth hormone mRNA in rat pituitary tumor cells.
Proc Natl Acad Sci U S A. 1977 May;74(5):2054-8 PMID: 266726
Rat insulin genes: construction of plasmids containing the coding sequences.
Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
Pregrowth hormone messenger RNA: glucocorticoid induction and identification in rat pituitary cells.
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2357-61 PMID: 267930
An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.
Cell. 1977 Sep;12(1):1-8 PMID: 902310
Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions.
Cell. 1977 Sep;12(1):9-21 PMID: 902321
Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.
Nature. 1977 Dec 8;270(5637):486-94 PMID: 339105
Thyroid and glucocorticoid hormones synergistically control growth hormone mRNA in cultured GH1 cells.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):45-9 PMID: 272661
Regulation of prolactin and somatotropin mRNAs by thyroliberin.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1294-8 PMID: 418408
Construction and identification by positive hybridization-translation of a bacterial plasmid containing a rat growth hormone structural gene sequence.
Nucleic Acids Res. 1978 Jun;5(6):2039-53 PMID: 353736
The isolation of structural genes from libraries of eucaryotic DNA.
Cell. 1978 Oct;15(2):687-701 PMID: 719759
Effect of estrogen on preprolactin messenger ribonucleic acid sequences.
Biochemistry. 1979 May 15;18(10):2044-8 PMID: 435463
Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.
Nucleic Acids Res. 1979 Mar;6(3):915-30 PMID: 375200
Organisation and sequences at the 5' end of a cloned complete ovalbumin gene.
Nature. 1979 Mar 29;278(5703):428-34 PMID: 450048
The ovalbumin gene: cloning and molecular organization of the entire natural gene.
Proc Natl Acad Sci U S A. 1979 May;76(5):2253-7 PMID: 287066
Improved methods for the formation and stabilization of R-loops.
Nucleic Acids Res. 1979 Jun 11;6(7):2499-317 PMID: 379821
The rat serum albumin gene: analysis of cloned sequences.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.8.4583

Published

1980-08-00

Pages

4583-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349888

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.8.4583 PMC Full Text View journal details

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Genomic organization of rat prolactin and growth hormone genes.

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