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PMID: 6254080 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5399-403

Anderson WF, Killos L, Sanders-Haigh L, Kretschmer PJ, Diacumakos EG

Abstract

A mixture of two recombinant plasmids was microinjected into mouse thymidine kinase-negative fibroblasts (L cells). One plasmid contained the thymidine kinase gene of herpes simplex virus type I and the other contained the human beta globin gene. Seven fibroblast colonies arising from injected cells incubated in hypoxanthine/aminopterin/thymidine medium were analyzed. These microinjected cells were shown to: (i) produce functionally active herpes simplex type I thymidine kinase enzyme, (ii) replicate the human beta globin gene, and (iii) produce human beta globin mRNA sequences at low levels. Thus, the genetic defect (lack of thymidine kinase activity) was corrected by the microinjected thymidine kinase gene, and a coinjected human beta globin gene was replicated and weakly expressed.

MeSH Terms
Animals Clone Cells Cloning, Molecular DNA, Recombinant/metabolism Escherichia coli/genetics Gene Expression Regulation Genetic Vectors Globins/genetics Humans L Cells/metabolism Mice Microinjections Nucleic Acid Hybridization Plasmids RNA, Messenger/biosynthesis Simplexvirus/genetics Thymidine Kinase/biosynthesis,genetics Transformation, Genetic
Chemicals
DNA, Recombinant RNA, Messenger Globins Thymidine Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Anderson W F
Killos L
Sanders-Haigh L
Kretschmer P J
Diacumakos E G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.9.5399
Published
1980-09-00
Pages
5399-403
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350066
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.9.5399 PMC Full Text View journal details
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