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PMID: 6254843 Published · ppublish English Journal Article

A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.

Gene ·Vol. 10 ·No. 3 ·1980-08-00 ·Pages 249-59

Loenen WA, Brammar WJ

Abstract

Lambda derivatives are described that can be used for cloning DNA fragments of about 20 kilobase pairs (kb) generated by restriction enzymes EcoRi, HindIII, BamHI, MboI and BglII. Recombinants can be selected by their Spi- phenotype and their propagation is facilitated by the presence of a chi site.

MeSH Terms
Bacteriophage lambda/genetics,growth & development Cloning, Molecular/methods DNA Restriction Enzymes Escherichia coli/genetics Genetic Vectors Molecular Weight Pseudomonas aeruginosa/genetics Recombination, Genetic Selection, Genetic Viral Interference
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Loenen W A
Brammar W J
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(80)90054-2
Published
1980-08-00
Pages
249-59
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
External Links
PubMed source DOI: 10.1016/0378-1119(80)90054-2 View journal details
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