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PMID: 6254852 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A cautionary note on the use of certain restriction endonucleases with methylated substrates.

Gene ·Vol. 11 ·No. 1-2 ·1980-10-00 ·Pages 169-71

Backman K

Abstract

Methylation of adenine and cytosine residues in DNA isolated from common strains of Escherichia coli K-12 can render that DNA resistant to cleavage by certain restriction endonucleases at those sites at which the recognition sequence for such an endonuclease overlaps (but does not include) a sequence recognized by methylases specified by the dam or dcm gene.

MeSH Terms
Adenine Cytosine DNA (Cytosine-5-)-Methyltransferases/genetics,metabolism DNA Restriction Enzymes/metabolism DNA, Bacterial/metabolism Escherichia coli/genetics Genes Methylation Substrate Specificity
Chemicals
DNA, Bacterial Cytosine DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes Adenine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Backman K
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(80)90097-9
Published
1980-10-00
Pages
169-71
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
Grants
NIADDK NIH HHS · AM-13894 · United States
NIGMS NIH HHS · GM-07446 · United States
External Links
PubMed source DOI: 10.1016/0378-1119(80)90097-9 View journal details
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