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PMID: 6255421 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA gyrase subunit stoichiometry and the covalent attachment of subunit A to DNA during DNA cleavage.

Nucleic acids research ·Vol. 8 ·No. 17 ·1980-09-11 ·Pages 3865-74

Sugino A, Higgins NP, Cozzarelli NR

Abstract

Escherichia coli DNA gyrase contains a 1:1 ratio of protomers coded by the genes gyrA and gyrB. This along with previous results shows that the enzyme has two copies of each protomer and thus a molecular weight of 400,000. Abortion of the gyrase reaction results in double-strand breakage of the DNA and covalent attachment of both gyrA protomers to the 5'-cut ends. We conclude that the gyrA protomer contains a critical part of the active site for the concerted breakage and reunion reaction of gyrase, the topoisomerase activity of the enzyme.

MeSH Terms
Binding Sites DNA Topoisomerases, Type II/metabolism DNA, Bacterial/metabolism Escherichia coli/enzymology Genes Macromolecular Substances Molecular Weight Operon Protein Binding
Chemicals
DNA, Bacterial Macromolecular Substances DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sugino A
Higgins N P
Cozzarelli N R
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.17.3865
Published
1980-09-11
Pages
3865-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324200
Subset
IM
Grants
NCI NIH HHS · CA-19265 · United States
NIGMS NIH HHS · GM-21397 · United States
External Links
PubMed source DOI: 10.1093/nar/8.17.3865 PMC Full Text View journal details
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