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PMID: 6255438 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A comparison of DNA cleavage by the restriction enzymes SalPI and PstI.

Nucleic acids research ·Vol. 8 ·No. 21 ·1980-11-11 ·Pages 4943-54

Carter JA, Chater KF, Bruton CJ, Brown NL

Abstract

Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions whose sequence is 5'-TGCA-3'. Bacteriophage R4G2 DNA, protected against SalPI cleavage by pregrowth on S. albus P, is also protected against PstI cleavage; and total DNA of both S. albus P and P. stuartii 164 is resistant to cleavage by both enzymes.

MeSH Terms
DNA/analysis DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type II Site-Specific Streptomyces/enzymology Substrate Specificity
Chemicals
DNA DNA Restriction Enzymes CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carter J A
Chater K F
Bruton C J
Brown N L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.21.4943
Published
1980-11-11
Pages
4943-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324271
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.21.4943 PMC Full Text View journal details
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