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PMID: 6255446 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Extraction of transcribing SV40 chromosomes in very low salt.

Nucleic acids research ·Vol. 8 ·No. 3 ·1980-02-11 ·Pages 573-86

Edenberg HJ

Abstract

SV40 chromosomes capable of continued RNA synthesis in vitro have been extracted from infected cells in solutions of very low ionic strength (5 mM HEPES, 0.25 mM MgCl2). The RNA made is of high molecular weight, and synthesis is sensitive to alpha-amanitin. More RNA is made than from previously described (high salt-extracted) transcription complexes. Transcribing SV40 chromosomes sediment at a rate intermediate between replicating and mature chromosomes, and have a higher ratio of protein to nucleic acid than either. They retain proteins that are lost during exposure to high salt, and might prove valuable in identifying proteins involved in SV40 transcription.

MeSH Terms
Ammonium Sulfate/pharmacology Chromosomes/analysis DNA-Directed RNA Polymerases/isolation & purification Methods RNA, Viral/biosynthesis Simian virus 40/genetics Temperature Transcription Factors/isolation & purification Transcription, Genetic
Chemicals
RNA, Viral Transcription Factors DNA-Directed RNA Polymerases Ammonium Sulfate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Edenberg H J
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.3.573
Published
1980-02-11
Pages
573-86
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327291
Subset
IM
Grants
NIGMS NIH HHS · GM-25681 · United States
External Links
PubMed source DOI: 10.1093/nar/8.3.573 PMC Full Text View journal details
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