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PMID: 6256259 Published · ppublish rus Journal Article

[Pseudomonas aeruginosa bacteriophages with DNA structure similar to the DNA structure of Mu1 phage. II. Evidence for similarity between D3112, B3, and B39 bacteriophages: analysis of DNA splits by restriction endonucleases, isolation of D3112 and B3 recombinant phages].

Bakteriofagi Pseudomonas aeruginosa, struktura DNA kotorykh skhodna so strukturoĭ DNK faga Mu1. Soobshchenie II. Dokazatel'stvo rodstva bakteriofagov D3112, B3 i B39: analiz rasshchepleniia DNK éndonukleazami restriktsii, vydelenie rekombinanta fagov D3112 i B3.

Genetika ·Vol. 16 ·No. 6 ·1980-00-00 ·Pages 975-84

Krylov VN, Bogush VG, Ianenko AS, Kirsanov NB

Abstract

It is found that bacteriophages B3 and B39 specific for Pseudomonas aeruginosa have the same genome structure as previously described phage D3112. On the right (S) end of their genomes a variable non-phage DNA is located (approximately 0.9-2.5 kilobases for different phages). It is probable that this variable DNa has its origin from different regions of bacterial chromosome. In genome of one of the phages, B3 phage, such variable DNA (not more than 150 base pairs) was found on the left end of DNA molecule. Isolation of a viable B3XD3112 recombinant phage and analysis of its genome with restriction technique and with studies of homo- and heteroduplex molecules had confirmed genetical relationship of B3 and D3112. Some essential non-homology of B3 and D3112 DNAs have been found on the right ends of genomes of the phages.

MeSH Terms
Bacteriophages/genetics DNA Restriction Enzymes DNA, Viral Genes, Viral Microscopy, Electron Mutation Nucleic Acid Heteroduplexes/analysis Pseudomonas aeruginosa/genetics Recombination, Genetic Species Specificity
Chemicals
DNA, Viral Nucleic Acid Heteroduplexes DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krylov V N
Bogush V G
Ianenko A S
Kirsanov N B
Article Info
Journal
Genetika
Abbr.
Genetika
ISSN
0016-6758
Published
1980-00-00
Pages
975-84
Language
rus
Region
Russia (Federation)
NLM ID
0047354
Subset
IM
External Links
PubMed source
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