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PMID: 6256607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ral gene of phage lambda. I. Identification of a non-essential gene that modulates restriction and modification in E. coli.

Molecular & general genetics : MGG ·Vol. 179 ·No. 1 ·1980-00-00 ·Pages 63-73

Zabeau M, Friedman S, Van Montagu M, Schell J

Abstract

Host controlled restriction in Escherichia coli can be relieved by pre-infecting restricting cells with modified lambda helper phages. This process, in which intact unmodified phage genomes are allowed to escape restriction attack, is mediated by a newly identified lambda function called ral. The ral gene has been located by deletion mapping between cIII and N. Efficient expression of the ral gene requires the product of the regulator gene N. Polyacrylamide gel analysis of the lambda proteins specified by the cIII-N region failed to reveal the product of the ral gene, but demonstrated that protein Ea10 is encoded by a gene located immediately to the left of ral. From these results the map order cIII-Ea10-ral-TL1-N was deduced. Ral specifically alleviates restriction in E. coli K and E. coli B, but does not affect restriction systems EcoRI, EcoRII and EcoP1. In addition, ral enhances the modification activity of the EcoK and EcoB restriction enzymes: we observed that efficient modification of progeny phages obtained by propagating unmodified lambda phages in r-m+ hosts, is dependent upon the presence of ral. We thus conclude that the ral gene product acts by modulating the restriction and modification activities of the type I restriction systems in E. coli, and the possible mechanisms will be discussed.

MeSH Terms
Bacteriophage lambda/genetics Chromosome Mapping DNA Restriction Enzymes/genetics DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Genes, Viral Genotype Phenotype
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zabeau M
Friedman S
Van Montagu M
Schell J
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48 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268447
Published
1980-00-00
Pages
63-73
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268447
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