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PMID: 6256732 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Construction of a flash-activated cyclic electron transport system by using bacterial reaction centers and the ubiquinone-cytochrome b-c1/c segment of mitochondria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6339-43

Packham NK, Tiede DM, Mueller P, Dutton PL

Abstract

Single-turnover electron transfer within the mitochondrial complex III has been studied by combining, in solution, the isolated complex from bovine heart with detergent-solubilized reaction centers of Rhodopseudomonas sphaeroides. Initiation of electron transfer by short flash activation resulted in the prompt oxidation of cytochrome c and reduction of cytochrome b. The subsequent reduction of ferricytochrome c was observed to be concomitant with the oxidation of the ferrocytochrome b, both reactions being inhibited by the addition of actimycin A. The rate of electron transfer through complex III is dependent upon the ambient redox potential poise in a way that is consistent with the presence of a redox component, presumably analogous to the photosynthetic ubiquinone Qz, which is an obligatory intermediate in electron transfer between cytochromes b and c. These results demonstrate cyclic electron transfer in a constructed assembly of mitochondrial complex III, cytochrome c, and photochemical reaction centers.

MeSH Terms

Animals Antimycin A/pharmacology Bacteriorhodopsins/metabolism Carotenoids/metabolism Cattle Cytochrome c Group/metabolism Cytochromes/metabolism Electron Transport/drug effects Light Mitochondria, Heart/metabolism Models, Biological Ubiquinone/metabolism

Chemicals

Cytochrome c Group Cytochromes Ubiquinone Carotenoids Bacteriorhodopsins Antimycin A

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Packham N K

Tiede D M

Mueller P

Dutton P L

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.11.6339

Published

1980-11-00

Pages

6339-43

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350279

Subset

Grants

NIGMS NIH HHS · GM 12202 · United States

NIGMS NIH HHS · GM 27309 · United States

External Links

PubMed source DOI: 10.1073/pnas.77.11.6339 PMC Full Text View journal details

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Construction of a flash-activated cyclic electron transport system by using bacterial reaction centers and the ubiquinone-cytochrome b-c1/c segment of mitochondria.

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