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PMID: 6257931 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloned human polyomavirus JC DNA can transform human amnion cells.

Journal of virology ·Vol. 36 ·No. 3 ·1980-12-00 ·Pages 878-82

Howley PM, Rentier-Delrue F, Heilman CA, Law MF, Chowdhury K, Israel MA, Takemoto KK

Abstract

The genome of the human polyomavirus JC (Mad-1 strain) was molecularly cloned in Escherichia coli by using the plasmid vector pBR322. Recombinant DNA molecules were constructed with the entire JC genome inserted either at its unique EcoRI site at 0.0 map units or at its unique BamHI site at 0.51 map units. Viral DNA from each of these recombinant plasmids was capable of transforming human amnion cells, and cell lines established from transformed foci were positive for JC tumor antigen as assayed by indirect immunofluorescence.

MeSH Terms

Amnion/cytology Cell Transformation, Viral Cells, Cultured Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes/metabolism DNA, Viral/genetics Escherichia coli/genetics Female Genes, Viral Genetic Vectors Humans Plasmids Polyomavirus/genetics

Chemicals

DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Howley P M

Rentier-Delrue F

Heilman C A

Law M F

Chowdhury K

Israel M A

Takemoto K K

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.36.3.878-882.1980

Published

1980-12-00

Pages

878-82

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC353715

Subset

Grants

NCI NIH HHS · CA06465 · United States

External Links

PubMed source DOI: 10.1128/JVI.36.3.878-882.1980 PMC Full Text View journal details

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