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PMID: 6259334 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sodium channel permeation in squid axons. I: Reversal potential experiments.

The Journal of physiology ·Vol. 307 ·1980-10-00 ·Pages 217-42

Begenisich TB, Cahalan MD

Abstract

1. Na channel reversal potentials were studied in perfused voltage clamped squid giant axons. The concentration dependence of ion selectivity was determined with both external and internal changes in Na and ammonium concentrations. 2. A tenfold change in the internal ammonium activity results in a 42 mV shift in the reversal potential, rather than the 56 mV shift expected from the Goldman, Hodgkin, Katz equation for a constant PNa/PNH4 ratio. However, changing [Na]o tenfold at constant internal [NH4] gives approximately the expected 56 mV shift. Therefore, the apparent channel selectivity depends upon the internal ammonium concentration but not the external Na concentration. 3. With ammonium outside and Na inside, the calculated permeability ratio is nearly constant, regardless of the permeant ion concentration. 4. Internal Cs ions can alter the Na/K permeability ratio. 5. The results are considered in terms of a three-barrier, two-site ionic permeation model.

MeSH Terms
Animals Axons/physiology Cell Membrane Permeability Decapodiformes In Vitro Techniques Ion Channels/physiology Kinetics Membrane Potentials/drug effects Models, Neurological Quaternary Ammonium Compounds/pharmacology Sodium/metabolism,pharmacology
Chemicals
Ion Channels Quaternary Ammonium Compounds Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Begenisich T B
Cahalan M D
References (37)
37 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1980.sp013432
Published
1980-10-00
Pages
217-42
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1283042
Subset
IM
Grants
NINDS NIH HHS · NS-14138 · United States
NINDS NIH HHS · NS00322 · United States
NINDS NIH HHS · NS14609 · United States
External Links
PubMed source DOI: 10.1113/jphysiol.1980.sp013432 PMC Full Text View journal details
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