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PMID: 6260756 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro.

Journal of bacteriology ·Vol. 146 ·No. 2 ·1981-05-00 ·Pages 668-75

Inouye H, Michaelis S, Wright A, Beckwith J

Abstract

The structural gene for alkaline phosphatase (phoA) of Escherichia coli was cloned into the PstI site of pBR322, from a transducing bacteriophage, lambda p(phoA-proC). The restriction map of the plasmid was established. Based upon this information, several phoA deletion plasmids as well as a smaller phoA+ plasmid were constructed. The genetic map and restriction map were correlated by recombination analysis. Cells carrying one of the phoA+ plasmids overproduce alkaline phosphatase 10-fold upon phosphate limitation. However, both regulation and processing of the enzyme were found to be normal.

MeSH Terms
Alkaline Phosphatase/genetics Bacteriophage lambda Cloning, Molecular DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific Escherichia coli/enzymology,genetics Genes Mutation Plasmids
Chemicals
DNA Restriction Enzymes CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific Alkaline Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Inouye H
Michaelis S
Wright A
Beckwith J
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23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.146.2.668-675.1981
Published
1981-05-00
Pages
668-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217011
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.146.2.668-675.1981 PMC Full Text View journal details
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