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PMID: 6260978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mapping of the viral mRNA encoding a super-T antigen of 115,000 daltons expressed in simian virus 40-transformed rat cell lines.

Journal of virology ·Vol. 37 ·No. 1 ·1981-01-00 ·Pages 24-35

May E, Kress M, Daya-Grosjean L, Monier R, May P

Abstract

Simian virus 40-transformed V11 F1 clone 1 subclone 7 rat cells produced a considerable amount of an elongated form of large-T antigen with an Mr of 115,000 (115K super-T antigen), but these cells did not produce detectable traces of normal-sized large-T antigen (86,000 daltons) (P. May, M. Kress, M. Lange, and E. May, Cold Spring Harbor Symp. Quant. Biol. 44:189-200, 1980). First, a comparison of the tryptic peptide fingerprints of 115K super-T and large-T antigens suggested that 115K super-T antigen is simian virus 40 coded and contains a duplication of amino acid sequences of large-T antigen. Second, from S1 mapping analysis of 115K super-T mRNA, performed with various restriction fragments of simian virus 40 DNA, it was concluded that super-T mRNA is a form of large-T mRNA containing a tandem duplication of the sequence extending from approximately 0.46 to 0.35 map unit. The duplicated sequence corresponded to that region of the simian virus 40 genome in which 12 of 13 tsA mutation sites are clustered (C. J. Lai and D. Nathans, Virology 66:70-81, 1975).

MeSH Terms
Animals Antigens, Neoplasm/genetics Antigens, Viral/genetics Antigens, Viral, Tumor Cell Line Cell Transformation, Viral Centrifugation, Density Gradient Chromatography, Thin Layer Chromosome Mapping Electrophoresis, Agar Gel Lung Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics Rats Repetitive Sequences, Nucleic Acid Simian virus 40/genetics,immunology
Chemicals
Antigens, Neoplasm Antigens, Viral Antigens, Viral, Tumor RNA, Messenger RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
May E
Kress M
Daya-Grosjean L
Monier R
May P
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.37.1.24-35.1981
Published
1981-01-00
Pages
24-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC170978
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.37.1.24-35.1981 PMC Full Text View journal details
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