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PMID: 6261684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.

Applied and environmental microbiology ·Vol. 41 ·No. 1 ·1981-01-00 ·Pages 255-60

Croxson MC, Bellamy AR

Abstract

A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.

MeSH Terms
Detergents Fatty Alcohols/pharmacology Feces/microbiology Humans Microbiological Techniques Reoviridae/isolation & purification Rotavirus/classification,isolation & purification Sodium Dodecyl Sulfate
Chemicals
Detergents Fatty Alcohols Sodium Dodecyl Sulfate dodecyl sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Croxson M C
Bellamy A R
References (22)
22 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.41.1.255-260.1981
Published
1981-01-00
Pages
255-60
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC243673
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.41.1.255-260.1981 PMC Full Text View journal details
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