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PMID: 6262792 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping a eukaryotic promoter: a DNA sequence required for in vivo expression of adenovirus pre-early functions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1381-5

Osborne TF, Schell RE, Burch-Jaffe E, Berget SJ, Berk AJ

Abstract

This study defines a DNA sequence upstream from the mRNA cap site required for in vivo expression of the adenovirus 2 pre-early region. The adenovirus 2 pre-early region and flanking sequences were cloned in Escherichia coli plasmid pBR322. Derivatives of the plasmid lacking portions of the upstream viral DNA sequence were constructed. An assay was devised to test the ability of these plasmid DNAs to complement an adenovirus 5 mutant with a deletion in the pre-early region. Plasmids that retained at least 38 base pairs upstream from the mRNA cap site had complementing activity similar to that of the original plasmid, which contains 229 base pairs of upstream viral sequence. However, plasmids retaining 23 or fewer base pairs of viral sequence upstream from the cap site had significantly reduced complementing activity. These results indicate that a portion of the adenovirus 2 sequence between 23 and 38 base pairs upstream from the mRNA cap site is required for expression of the pre-early region. This interval includes the Goldberg-Hogness box-like sequence T-A-T-T-T-A-T-A.

MeSH Terms
Adenoviruses, Human/genetics,metabolism Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Genetic Complementation Test HeLa Cells/metabolism Humans Plasmids RNA Caps/genetics Transcription, Genetic Transfection
Chemicals
DNA, Viral RNA Caps DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Osborne T F
Schell R E
Burch-Jaffe E
Berget S J
Berk A J
References (39)
39 references, click to expand
  1. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.
    Nature. 1980 Jun 5;285(5764):367-73 PMID: 7383157
  2. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6 PMID: 6929494
  3. Regulatory mutants of polyoma virus defective in DNA replication and the synthesis of early proteins.
    Cell. 1980 Jun;20(2):401-9 PMID: 6248242
  4. Expression of unselected adenovirus genes in human cells co-transformed with the HSV-1 tk gene and adenovirus 2 DNA.
    Cell. 1980 Sep;21(2):453-63 PMID: 6250722
  5. Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses.
    Nature. 1979 Oct 25;281(5733):694-6 PMID: 551290
  6. Biogenesis, structures, and sites of encoding of the 5' termini of adenovirus-2 mRNAs.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:415-28 PMID: 6933038
  7. Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3865-9 PMID: 6253995
  8. Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease.
    Nucleic Acids Res. 1980 Feb 11;8(3):543-54 PMID: 6255444
  9. DNA sequence required for initiation of transcription in vitro from the major late promoter of adenovirus 2.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):820-4 PMID: 6262775
  10. A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.
    Plasmid. 1980 Jan;3(1):88-91 PMID: 6278527
  11. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  12. Extracellular nucleases of Pseudomonas BAL 31. I. Characterization of single strand-specific deoxyriboendonuclease and double-strand deoxyriboexonuclease activities.
    Nucleic Acids Res. 1975 Sep;2(9):1459-92 PMID: 1178526
  13. Autoregulation of simian virus 40 gene A by T antigen.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3083-7 PMID: 184459
  14. The infectivity of adenovirus 5 DNA-protein complex.
    Virology. 1976 Dec;75(2):442-56 PMID: 1032904
  15. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.
    Virology. 1977 Mar;77(1):319-29 PMID: 841862
  16. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  17. Identification of a protein linked to the ends of adenovirus DNA.
    Cell. 1977 Jun;11(2):283-95 PMID: 196758
  18. Ultraviolet mapping of the adenovirus 2 early promoters.
    Cell. 1977 Sep;12(1):45-55 PMID: 902320
  19. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  20. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  21. Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.
    Cell. 1978 Dec;15(4):1463-75 PMID: 729003
  22. RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):160-4 PMID: 284328
  23. Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside.
    J Bacteriol. 1979 Apr;138(1):270-2 PMID: 374372
  24. Mapping of RNA initiation sites by high doses of uv irradiation: evidence for three independent promoters within the left 11% of the Ad-2 genome.
    Virology. 1979 Apr 15;94(1):175-84 PMID: 442530
  25. Adenovirus 5 DNA sequences present and RNA sequences transcribed in transformed human embryo kidney cells (HEK-Ad-5 or 293).
    Virology. 1979 Apr 30;94(2):460-9 PMID: 452423
  26. Structural analysis of the fibroin gene at the 5' end and its surrounding regions.
    Cell. 1979 Feb;16(2):425-36 PMID: 455439
  27. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
    Cell. 1979 Jul;17(3):683-9 PMID: 476833
  28. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  29. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  30. The nucleotide sequence of the transforming HindIII-G fragment of adenovirus type 5 DNA. The region between map positions 4.5 (HpaI site) and 8.0 (HindIII site).
    Gene. 1979 May;6(1):75-90 PMID: 478299
  31. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
    Cell. 1979 Aug;17(4):935-44 PMID: 487437
  32. An adenovirus type 5 early gene function regulates expression of other early viral genes.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3665-9 PMID: 291030
  33. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  34. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  35. Complex splicing patterns of RNAs from the early regions of adenovirus-2.
    J Mol Biol. 1979 Oct 25;134(2):265-303 PMID: 537065
  36. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  37. Colony hybridization.
    Methods Enzymol. 1979;68:379-89 PMID: 542125
  38. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  39. E. coli RNA polymerase interacts homologously with two different promoters.
    Cell. 1980 Jun;20(2):269-81 PMID: 6248238
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1381
Published
1981-03-00
Pages
1381-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319134
Subset
IM
Grants
NCI NIH HHS · CA 16042-05 · United States
NCI NIH HHS · CA 25235 · United States
NCRR NIH HHS · RR0-7009 · United States
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1073/pnas.78.3.1381 PMC Full Text View journal details
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