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PMID: 6262803 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a cDNA clone coding for the beta chain of bovine fibrinogen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1466-70

Chung DW, Rixon MW, MacGillivray RT, Davie EW

Abstract

Recombinant plasmids containing bovine cDNA have been screened with a radiolabeled cDNA enriched for bovine fibrinogen. A number of plasmids containing cDNAs for fibrinogen were identified by this assay. One plasmid, designated pBI beta 1, was found to contain a cDNA insert of 1372 base pairs. The sequence of the cDNA insert for this plasmid was then determined. It was shown to code for 424 amino acids of the beta chain of fibrinogen, starting with residue 44. This and other data made it possible to construct the complete amino acid sequence of the beta chain of the protein. Comparison of the amino acid sequence of the beta chain of bovine fibrinogen with the corresponding chain of the human molecule indicated that the two chains are greater than 80% homologous.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Fibrinogen/genetics Genetic Code Humans Macromolecular Substances Nucleic Acid Hybridization Plasmids Species Specificity
Chemicals
DNA, Recombinant Macromolecular Substances Fibrinogen DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chung D W
Rixon M W
MacGillivray R T
Davie E W
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1466
Published
1981-03-00
Pages
1466-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319151
Subset
IM
Grants
NHLBI NIH HHS · HL 16919 · United States
Databases
GENBANK
J00006
External Links
PubMed source DOI: 10.1073/pnas.78.3.1466 PMC Full Text View journal details
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