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PMID: 6262815 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tissue-specific exposure of chromatin structure at the 5' terminus of the rat preproinsulin II gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1577-80

Wu C, Gilbert W

Abstract

The 5' terminus of the rat preproinsulin II gene exhibits a tissue-dependent DNase I sensitivity. Only in the chromatin froma pancreatic beta-cell tumor, but not in liver, spleen, kidney, or brain chromatin, is a region at and before the 5' end of the gene exposed to cleavage. The region of exposure extends 250-300 base pairs upstream from the 5' terminus of the preproinsulin mRNA. Such a region may allow control sequences special access to regulatory proteins.

MeSH Terms
Adenoma, Islet Cell/metabolism Animals Chromatin/metabolism,ultrastructure DNA/genetics DNA Restriction Enzymes Deoxyribonuclease I Deoxyribonucleases Endonucleases Genes Insulin Molecular Weight Neoplasms, Experimental/metabolism Organ Specificity Pancreatic Neoplasms/metabolism Proinsulin/genetics Protein Precursors/genetics Rats
Chemicals
Chromatin Insulin Protein Precursors preproinsulin DNA Proinsulin Deoxyribonucleases Endonucleases DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wu C
Gilbert W
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1577
Published
1981-03-00
Pages
1577-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319174
Subset
IM
Grants
NIGMS NIH HHS · GM21514-06 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1577 PMC Full Text View journal details
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