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PMID: 6263223 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The influence of divalent cations on the stability of human rotavirus.

Archives of virology ·Vol. 67 ·No. 1 ·1981-00-00 ·Pages 1-9

Shirley JA, Beards GM, Thouless ME, Flewett TH

Abstract

The influence of divalent cations on the stability of human rotavirus was investigated using the indirect immunofluorescence (FA) technique in LLC-MK2 cells to titrate infectivity. Rotavirus infectivity was stabilized by calcium and strontium ions in solution but not by magnesium ions. Rotavirus isolates were found to be much less stable at 37 degrees C than at + 4 degrees C or 20 degrees. A study of virus morphology at intervals during the course of the experiment and treatment with the chelating agents EDTA and EGTA suggests that loss of infectivity coincides with the removal of the outer capsid layer and that calcium may be required to maintain virus integrity.

MeSH Terms
Animals Calcium/pharmacology Cations, Divalent/pharmacology Cell Line Child, Preschool Diarrhea/microbiology Edetic Acid/pharmacology Egtazic Acid/pharmacology Feces/microbiology Haplorhini Humans Lung Magnesium/pharmacology Reoviridae/pathogenicity Rotavirus/pathogenicity Strontium/pharmacology
Chemicals
Cations, Divalent Egtazic Acid Edetic Acid Magnesium Calcium Strontium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shirley J A
Beards G M
Thouless M E
Flewett T H
References (10)
10 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01314596
Published
1981-00-00
Pages
1-9
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01314596 View journal details
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