Inhibition of 2',3'-cyclic nucleotide 3'-phosphodiesterase activity from bovine, human and guinea pig brain.

The purified oligodendrocyte enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) obtained from bovine, human and guinea pig brain was inhibited by theophylline, caffeine, cupric chloride and organomercurials using a spectrophotometric assay and 1 mM 2',3'- cyclic cytidylate as the substrate. The observed inhibition by theophylline, caffeine and organomercurials was reversible.