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PMID: 6263862 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501.

Journal of bacteriology ·Vol. 147 ·No. 1 ·1981-07-00 ·Pages 110-7

Pemberton JM, Bowen AR

Abstract

Insertion of the mercury resistance transposon Tn501 into broad-host-range plasmid RP1 greatly enhanced the ability of this plasmid to promote chromosome transfer in the photosynthetic bacterium Rhodopseudomonas sphaeroides. Compared with the wild-type RP1, which produced less than 10(-8) recombinants per donor cell, RP1::Tn501 produced between 10(-3) and 10(-7) recombinants per donor cell depending upon the marker selected. Plasmid RP1::Tn501 promoted polarized transfer of the chromosome from one or perhaps two origins on the chromosome, giving rise to two linkage groups. All of the biosynthetic and antibiotic resistance genes that have been mapped, including those involved in photosynthesis, occur on one or another of these linkage groups.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacteriochlorophylls/genetics Carotenoids/genetics Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic DNA Transposable Elements Genetic Linkage R Factors Recombination, Genetic Rhodobacter sphaeroides/genetics
Chemicals
Anti-Bacterial Agents Bacteriochlorophylls DNA Transposable Elements Carotenoids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pemberton J M
Bowen A R
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.1.110-117.1981
Published
1981-07-00
Pages
110-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216014
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.147.1.110-117.1981 PMC Full Text View journal details
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