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PMID: 6264397 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of cloned human DNA fragments carrying reiterated sequences common to both autosomes and the X chromosome.

Nucleic acids research ·Vol. 9 ·No. 8 ·1981-04-24 ·Pages 1853-72

Schmeckpeper BJ, Willard HF, Smith KD

Abstract

Several recombinants were identified and purified from a cloned library of human DNA by virtue of their homology to DNA from a mouse-human hybrid cell line containing a single human chromosome, the X, and their lack of homology to mouse DNA. Three recombinants were characterized in detail, and all were homologous to reiterated DNA from the human X chromosome. These recombinants also were homologous to reiterated sequences on one or more human autosomes and, therefore, were not X chromosome specific. The recombinant DNA fragments homologous to human reiterated X DNA were the same fragments homologous to human reiterated autosomal DNA. Digestion of genomic DNAs with several restriction enzymes revealed that the pattern of fragments homologous to one recombinant, lambda Hb2, was the same on autosomes as on the X chromosome, suggesting that the molecular organization of these elements on the X is not distinct from their organization on autosomes.

MeSH Terms
Animals Cell Line Chromosomes, Human/analysis Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/isolation & purification Female Humans Hybrid Cells/analysis Mice Molecular Weight Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Sex Chromosomes/analysis X Chromosome/analysis
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schmeckpeper B J
Willard H F
Smith K D
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.8.1853
Published
1981-04-24
Pages
1853-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326808
Subset
IM
Grants
NIGMS NIH HHS · GM07471 · United States
NHLBI NIH HHS · HL15026 · United States
NCRR NIH HHS · RR-5378 · United States
External Links
PubMed source DOI: 10.1093/nar/9.8.1853 PMC Full Text View journal details
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