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PMID: 6264442 Published · ppublish English Journal Article

Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 1 ·1981-01-00 ·Pages 420-4

Greenberg HB, Kalica AR, Wyatt RG, Jones RW, Kapikian AZ, Chanock RM

Abstract

Fastidious human rotaviruses that did not undergo productive infection in tissue culture were rescued by genetic reassortment during mixed infection with a temperature-sensitive (ts) mutant of a cultivatable bovine rotavirus. In this manner, the genes of the fastidious rotavirus that restricted growth in vitro were replaced by the corresponding genes from a tissue culture-adapted rotavirus. We recovered genetically reassorted viruses that grew to high titer and were neutralized specifically by hyperimmune guinea pig type 1 or type 2 human rotavirus antiserum. Preliminary RNA analysis of these clones disclosed that they were indeed viruses with reassorted genes.

MeSH Terms
Animals Chlorocebus aethiops Kidney Mutation Neutralization Tests Recombination, Genetic Reoviridae/growth & development Rotavirus/genetics,growth & development,immunology Temperature Virus Cultivation
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Greenberg H B
Kalica A R
Wyatt R G
Jones R W
Kapikian A Z
Chanock R M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.1.420
Published
1981-01-00
Pages
420-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319065
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.1.420 PMC Full Text View journal details
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